Li Hui-ming, Lu Shi-duo, Xia Xin, Xu Ping, Wang Feng, Huang Qian
Central Experimental Laboratory, The First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.
Zhonghua Yan Ke Za Zhi. 2005 Feb;41(2):119-22.
To investigate the protective effects of transplantation of cell line stably expressing and secreting human ciliary neurotrophic factor (hCNTF) on the degeneration of retinal ganglion cells (RGCs) after optic nerve transection.
Plasmid encoding hCNTF was transfected into human lung fibroblast (HLF) cell line, then the stably transfected clones were selected with methopterin. In adult SD rats, RGCs were labeled with retrograde axonal tracer fluorogold (FG) injected to their targets including dorsal lateral geniculate nuclei (dLGN) and superior colliculi (SC). Seven days later, the optic nerve was transected alone or in combination with transplantation of HLF cells. Five, 14, 17, 21 and 28 days after axotomy, the retinas were mounted and examined under fluorescence microscope to observe the RGCs.
Compared to the controls, the density of RGC in axotomy group decreased by 67.44% and 82.73% on the 14th and 28th day, respectively. In the eyes with hCNTF-transfected HLF cells transplantation, RGC density was higher than that of the axotomy group on the 5th, 17th, 21st day after axotomy (P < 0.05). On the 5th day, the morphology of RGC in the hCNTF group remained the same as the controls, whereas the morphology of RGC in the axotomy alone group began to change.
hCNTF administered at the time of optic nerve transection can protect RGC from degeneration, increasing the numbers of surviving RGCs and delaying the death of RGCs.
探讨移植稳定表达并分泌人睫状神经营养因子（hCNTF）的细胞系对视神经横断后视网膜神经节细胞（RGCs）退变的保护作用。
将编码hCNTF的质粒转染到人肺成纤维细胞（HLF）系，然后用甲氨蝶呤筛选稳定转染的克隆。在成年SD大鼠中，将逆行轴突示踪剂荧光金（FG）注入包括背外侧膝状核（dLGN）和上丘（SC）在内的靶点，标记RGCs。7天后，单独横断视神经或联合移植HLF细胞。轴突切断后5、14、17、21和28天，将视网膜固定并在荧光显微镜下检查以观察RGCs。
与对照组相比，轴突切断组RGC密度在第14天和第28天分别下降了67.44%和82.73%。在移植了转染hCNTF的HLF细胞的眼中，轴突切断后第5、17、21天RGC密度高于轴突切断组（P<0.05）。第5天，hCNTF组RGC的形态与对照组相同，而单纯轴突切断组RGC的形态开始改变。
视神经横断时给予hCNTF可保护RGC免于退变，增加存活RGC的数量并延缓RGC的死亡。
