Wang Li, Zhao Chun-Mei, Wang Yi-Ju, Liu Jian
College of Life Sciences, Shandong Normal University, Jinan 250014, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2005 Apr;31(2):167-74.
There are many reports about the correlation between small molecular heat-shock protein (sHSP) and the acquirement of chilling tolerance, but no direct evidence that sHSP confers enhanced chilling tolerance to plant has been reported. A DNA construct, including tomato chloroplast-localized small molecular heat-shock protein (CPsHSP) cDNA under the control of cauliflower mosaic virus 35S (35SCaMV) promoter, was introduced into the genome of tomato plants. The chilling tolerance of the transgenic tomato lines and the non-transgenic tomato was evaluated. After exposure to chilling stress, the transgenic plants exhibited lighter chilling-injured symptoms, suffered less electrolyte leakage and less destruction of chlorophyll, accumulated less anthocyanins and less MDA and kept higher value of net photosynthetic rate, than non-transgenic plant. All results indicated consistently that transgenic tomato plants had stronger chilling tolerance. These characters are ascribed to constitutive expression of cpshsp and lead to the conclusion that HSP can enhance chilling tolerance in plant.
关于小分子热激蛋白（sHSP）与植物耐寒性获得之间的相关性已有许多报道，但尚未有直接证据表明sHSP能赋予植物更强的耐寒性。将一个DNA构建体导入番茄植株基因组，该构建体包含在花椰菜花叶病毒35S（35SCaMV）启动子控制下的番茄叶绿体定位小分子热激蛋白（CPsHSP）cDNA。对转基因番茄株系和非转基因番茄的耐寒性进行了评估。与非转基因植株相比，转基因植株在遭受低温胁迫后，表现出较轻的冷害症状，电解质渗漏较少，叶绿素破坏较少，花青素和丙二醛积累较少，净光合速率保持较高水平。所有结果一致表明，转基因番茄植株具有更强的耐寒性。这些特性归因于cpshsp的组成型表达，从而得出HSP可以增强植物耐寒性的结论。
