Bharati Kaushik, Appaiahgari Mohan Babu, Vrati Sudhanshu
National Institute of Immunology, New Delhi, India.
Microbiol Immunol. 2005;49(4):349-53. doi: 10.1111/j.1348-0421.2005.tb03739.x.
We have previously shown that immunization of mice with plasmid pMEa synthesizing Japanese encephalitis virus (JEV) envelope protein induced anti-JEV humoral and cellular immune responses. We now show that intra-muscular co-administration of mice with pMEa and pGM-CSF, encoding murine granulocyte-macrophage colony-stimulating factor or pIL-2, encoding murine interleukin-2 given 4 days after pMEa, augmented anti-JEV antibody titers. This did not enhance the level of protection in immunized mice against JEV. However, intra-dermal co-administration of pMEa and pGM-CSF in mice using the gene gun, enhanced anti-JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge.
我们之前已经表明，用合成日本脑炎病毒（JEV）包膜蛋白的质粒pMEa免疫小鼠可诱导抗JEV体液免疫和细胞免疫反应。我们现在表明，在pMEa给药4天后，将pMEa与编码小鼠粒细胞-巨噬细胞集落刺激因子的pGM-CSF或编码小鼠白细胞介素-2的pIL-2肌肉内共同给予小鼠，可提高抗JEV抗体滴度。这并未增强免疫小鼠对JEV的保护水平。然而，使用基因枪在小鼠皮内共同给予pMEa和pGM-CSF，可提高抗JEV抗体滴度，从而提高小鼠对致死性JEV攻击的保护水平。
