Han Bo, Zhou Gengyin, Zhang Qinghui, Zhang Jing, Wang Xiaojuan, Tang Weihua, Kakudo Kennichi
Department of Pathology, Shandong University, Jinan city, Shandong, China.
J Exp Ther Oncol. 2004 Dec;4(4):335-42.
Arsenic trioxide (ATO) has been established to be an effective agent for treating newly diagnosed and relapsed acute promyelocytic leukemia (APL) patients. Laboratory data suggest that ATO induces apoptosis of hematopoietic or several solid tumor cells. However, to date, its effect on lung carcinoma has not been fully explored. In the present study, we investigated the effect of ATO on human lung carcinoma PG cells in vitro. We found ATO significantly inhibited the proliferation of PG cells in a dose- and time-dependent manner. ATO-induced apoptosis of PG cells was confirmed by the observance of typical morphological changes and detected by the analysis of flow cytometry (FCM). ATO significantly inhibited Bcl-2 and Pgp expression of PG cells by SABC immunohistochemistry and FCM analysis. In conclusion, our findings indicated that ATO induced apoptosis of PG cells and down-regulation of Bcl-2 and Pgp expressions, and these data might provide some theoretical basis for its clinical use in treating lung carcinoma.
三氧化二砷（ATO）已被证实是治疗新诊断及复发的急性早幼粒细胞白血病（APL）患者的有效药物。实验室数据表明，ATO可诱导造血细胞或多种实体瘤细胞凋亡。然而，迄今为止，其对肺癌的作用尚未得到充分研究。在本研究中，我们在体外研究了ATO对人肺癌PG细胞的作用。我们发现ATO以剂量和时间依赖性方式显著抑制PG细胞的增殖。通过观察典型的形态学变化证实了ATO诱导PG细胞凋亡，并通过流式细胞术（FCM）分析进行检测。通过SABC免疫组织化学和FCM分析，ATO显著抑制PG细胞的Bcl-2和Pgp表达。总之，我们的研究结果表明，ATO诱导PG细胞凋亡并下调Bcl-2和Pgp表达，这些数据可能为其在肺癌治疗中的临床应用提供一些理论依据。
