Sánchez L, Coveñas R, Aguirre J A, Narváez J A, Gómez A, Tramu G
Institute of Neurosciences of Castilla and León, Laboratory of Neuroanatomy of the Peptidergic System, 37007 Salamanca, Spain.
Arch Ital Biol. 2005 Feb;143(1):29-50.
Using an indirect immunoperoxidase technique, the location of cell bodies and fibers containing substance P, neurokinin A and neurokinin B was studied in the cat spinal cord. The former two neuropeptides showed a widespread distribution throughout the whole spinal cord, whereas the distribution of neurokinin B was more restricted. Neurokinin A-immunoreactive structures showed a more widespread distribution and a higher density than the immunoreactive structures observed to contain substance P. In the cat spinal cord, we observed cell bodies containing neurokinin A, but no cell bodies containing neurokinin B or substance P were found. These cell bodies were located in laminae V (sacral 1 and 2 levels), VI (sacral 1 and 3), VII (lumbar 7, sacral 1 and 3, caudal 1) and X (sacral 1). Laminae I and II showed the highest density of immunoreactive fibers for each of the three tachykinins studied, being in general lamina IV who showed the lowest number of immunoreactive fibers containing substance P, neurokinin A or B. The anatomical distribution of the three tachykinins studied in the cat spinal cord indicates that the neuropeptides could be involved in the neurotransmission and/or in the neuromodulation of nociceptive information, as well as in autonomic and affective responses to pain. Moreover, the involvement of substance P, neurokinin A or B in other functions unrelated to the transmission of pain is also possible (autonomic and motor functions). The distribution of the neuropeptides studied in the cat is compared with the location of the same neuropeptides in the spinal cord of other species. The possible origin of the tachykinergic fibers in the cat spinal cord is also discussed.
采用间接免疫过氧化物酶技术，研究了猫脊髓中含P物质、神经激肽A和神经激肽B的细胞体和纤维的定位。前两种神经肽在整个脊髓中广泛分布，而神经激肽B的分布则更为局限。与含P物质的免疫反应性结构相比，神经激肽A免疫反应性结构分布更广泛，密度更高。在猫脊髓中，我们观察到了含神经激肽A的细胞体，但未发现含神经激肽B或P物质的细胞体。这些细胞体位于第V层（骶1和2节段）、第VI层（骶1和3节段）、第VII层（腰7、骶1和3节段、尾1节段）和第X层（骶1节段）。在所研究的三种速激肽中，第I层和第II层显示出最高密度的免疫反应性纤维，而一般来说，第IV层显示含P物质、神经激肽A或B的免疫反应性纤维数量最少。对猫脊髓中研究的三种速激肽的解剖分布表明，这些神经肽可能参与伤害性信息的神经传递和/或神经调节，以及对疼痛的自主和情感反应。此外，P物质、神经激肽A或B也可能参与与疼痛传递无关的其他功能（自主和运动功能）。将猫中研究的神经肽分布与其他物种脊髓中相同神经肽的定位进行了比较。还讨论了猫脊髓中速激肽能纤维的可能起源。
