Fujita K, Tsujimura A, Takao T, Miyagawa Y, Matsumiya K, Koga M, Takeyama M, Fujioka H, Aozasa K, Okuyama A
Department of Urology and Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita Osaka 565-0871, Japan.
Hum Reprod. 2005 Aug;20(8):2289-94. doi: 10.1093/humrep/dei032. Epub 2005 Apr 21.
Microdissection testicular sperm extraction (TESE) has provided new hope for successful sperm retrieval to patients with Sertoli cell-only syndrome (SCO). We determined expression of the inhibin alpha subunit, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in Sertoli cells obtained from patients with SCO immunohistochemically and compared expression rates with rates of microdissection TESE sperm retrieval.
Testicular biopsy specimens were obtained from 52 men with non-obstructive azoospermia who underwent microdissection TESE and were diagnosed with SCO by histological analysis.
All specimens showed intense staining for the inhibin alpha subunit. Moderate or intense staining for GDNF was observed in 65.8% of specimens. All but one showed moderate or intense staining for SCF. Among specimens negative for GDNF, the sperm retrieval rate was significantly higher (100%) for specimens with intense staining for SCF than for specimens with no or moderate staining (30.7%) (P<0.05) for SCF.
GDNF expression differs among patients with SCO. The sperm retrieval rate was high in cases of no staining for GDNF and intense staining for SCF.
显微切割睾丸精子提取术（TESE）为唯支持细胞综合征（SCO）患者成功获取精子带来了新希望。我们采用免疫组织化学方法测定了SCO患者支持细胞中抑制素α亚基、胶质细胞源性神经营养因子（GDNF）和干细胞因子（SCF）的表达，并将表达率与显微切割TESE精子获取率进行了比较。
从52例非梗阻性无精子症患者获取睾丸活检标本，这些患者接受了显微切割TESE，并经组织学分析诊断为SCO。
所有标本的抑制素α亚基均呈强染色。65.8%的标本中观察到GDNF呈中度或强染色。除1例标本外，其余所有标本的SCF均呈中度或强染色。在GDNF阴性的标本中，SCF强染色的标本精子获取率（100%）显著高于SCF无染色或中度染色的标本（30.7%）（P<0.05）。
SCO患者中GDNF表达存在差异。GDNF无染色且SCF强染色的病例精子获取率较高。
