Rooney Henrietta C E, Van't Klooster John W, van der Hoorn Renier A L, Joosten Matthieu H A J, Jones Jonathan D G, de Wit Pierre J G M
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK.
Science. 2005 Jun 17;308(5729):1783-6. doi: 10.1126/science.1111404. Epub 2005 Apr 21.
How plants recognize pathogens and activate defense is still mysterious. Direct interaction between pathogen avirulence (Avr) proteins and plant disease resistance proteins is the exception rather than the rule. During infection, Cladosporium fulvum secretes Avr2 protein into the apoplast of tomato leaves and, in the presence of the extracellular leucine-rich repeat receptor-like Cf-2 protein, triggers a hypersensitive response (HR) that also requires the extracellular tomato cysteine protease Rcr3. We show here that Avr2 binds and inhibits Rcr3 and propose that the Rcr3-Avr2 complex enables the Cf-2 protein to activate an HR.
植物如何识别病原体并激活防御机制仍是个谜。病原体无毒（Avr）蛋白与植物抗病蛋白之间的直接相互作用是例外而非普遍规律。在感染过程中，番茄叶霉病菌将Avr2蛋白分泌到番茄叶片的质外体中，在细胞外富含亮氨酸重复序列的类受体Cf-2蛋白存在的情况下，引发超敏反应（HR），这一过程还需要细胞外番茄半胱氨酸蛋白酶Rcr3。我们在此表明，Avr2结合并抑制Rcr3，并提出Rcr3-Avr2复合物使Cf-2蛋白能够激活超敏反应。
