Kendall C W, Janezic S A, Friday D, Rao A V
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Ontario, Canada.
Nutr Cancer. 1992;17(2):107-14. doi: 10.1080/01635589209514178.
The effect of dietary cholesterol on the development of colonic preneoplastic aberrant crypts, as well as its influence on the proliferative status of the intestinal epithelium, was investigated in mice exposed to the chemical carcinogen azoxymethane. Two strains of mice, C57BL/6J and BALB/cJ, were fed a semisynthetic diet containing 0% (control), 1.25%, or 5.00% cholesterol for eight weeks. During the first four weeks of the experiment, mice were given weekly injections of azoxymethane. Cholesterol supplementation significantly increased the formation of aberrant crypts (p less than 0.0001), enhanced the rate of cell proliferation (p less than 0.0001), altered the cell proliferative pattern, and increased crypt height (p less than 0.05) and the total number of cells per crypt (p less than 0.01) in the colonic epithelium of both mouse strains. C57BL/6J mice developed a greater number of aberrant crypts (p less than 0.0001). However, a diet-strain interaction was not observed. The results of this study indicate that dietary cholesterol enhances colon carcinogenesis in the murine colon and therefore may be an important factor in the etiology of large bowel cancer in humans.
在接触化学致癌物氧化偶氮甲烷的小鼠中，研究了膳食胆固醇对结肠癌前异常隐窝形成的影响及其对肠上皮细胞增殖状态的影响。给C57BL/6J和BALB/cJ两种品系的小鼠喂食含0%（对照）、1.25%或5.00%胆固醇的半合成饮食，持续八周。在实验的前四周，每周给小鼠注射氧化偶氮甲烷。补充胆固醇显著增加了异常隐窝的形成（p<0.0001），提高了细胞增殖率（p<0.0001），改变了细胞增殖模式，并增加了两种小鼠品系结肠上皮中隐窝高度（p<0.05）和每个隐窝的细胞总数（p<0.01）。C57BL/6J小鼠形成的异常隐窝数量更多（p<0.0001）。然而，未观察到饮食-品系相互作用。本研究结果表明，膳食胆固醇会增强小鼠结肠的结肠癌发生，因此可能是人类大肠癌病因中的一个重要因素。
