Xie Hua, Xue Yan-fen, Zhao Ai-min, Li Tie-gang, Ma Yan-he
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wei Sheng Wu Xue Bao. 2005 Feb;45(1):1-5.
The environmental DNA was directly extracted from the sediment in Parece Vela Basin, Pacific Ocean, at a depth of 5010 m. Bacterial 16S rRNA gene library of 32 clones was generated using bacterial universal primers and 16S rDNA sequences were analyzed phylogenetically. 17 phylotypes were obtained. The library was dominated by gamma-Proteobacteria, alpha-Proteobacteria and marine uncultured bacteria. Sixty-two percent of the cloned sequences was highly related to the known bacteria in the genus Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, Pseudoalteromonas (> 96% sequence similarity), while some of the cloned sequences showed less affiliation with known taxa (< 94% sequence similarity) and may represent novel taxa.
从太平洋帕里西维拉海盆5010米深处的沉积物中直接提取环境DNA。使用细菌通用引物构建了一个包含32个克隆的细菌16S rRNA基因文库，并对16S rDNA序列进行了系统发育分析。共获得17个系统发育型。该文库以γ-变形菌纲、α-变形菌纲和海洋未培养细菌为主。62%的克隆序列与盐单胞菌属、食烷菌属、假单胞菌属、不动杆菌属、假交替单胞菌属中的已知细菌高度相关（序列相似性>96%），而一些克隆序列与已知分类群的亲缘关系较低（序列相似性<94%），可能代表新的分类群。
