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[从痰液中分离分枝杆菌。MB检测与放射性测量法和传统方法的比较]

[Isolation of mycobacteria from sputum. Comparison of the MB Check with the radiometric and conventional method].

作者信息

Rüsch-Gerdes S, Jährling A

机构信息

Forschungsinstitut Borstel.

出版信息

Pneumologie. 1992 Mar;46(3):107-10.

PMID:1584735
Abstract

1179 sputum samples were investigated by the MB-Check, the conventional method and Bactec 460 to compare the recovery rate of Mycobacterium tuberculosis, the contamination rate, and time requirement for the isolation. Of the 293 positive sputa, the MB-Check detected 89,1%, Löwenstein Jensen medium 90,1%, the Bactec-system 90,4%, the combination MB-Check/Löwenstein Jensen 95,9%; and the combination Bactec/Löwenstein Jensen 96,6%. The average detection times for 130 Mycobacterium tuberculosis-strains with the MB-Check slide, with the liquid medium of the MB-Check, with Löwenstein Jensen and with Bactec were 26.0, 20.4, 25.3, and 12.0 days, respectively.

摘要

对1179份痰液样本采用MB-Check、传统方法和Bactec 460进行检测,以比较结核分枝杆菌的回收率、污染率及分离所需时间。在293份阳性痰液中,MB-Check检测出89.1%,罗氏培养基检测出90.1%,Bactec系统检测出90.4%,MB-Check/罗氏培养基联合检测出95.9%;Bactec/罗氏培养基联合检测出96.6%。对于130株结核分枝杆菌菌株,使用MB-Check载玻片、MB-Check液体培养基、罗氏培养基和Bactec检测的平均时间分别为26.0天、20.4天、25.3天和12.0天。

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