Gebicke-Haerter Peter
Department of Psychopharmacology, Central Institute for Mental Health, University of Heidelberg, Mannheim, Germany.
Addict Biol. 2005 Mar;10(1):37-46. doi: 10.1080/13556210412331327812.
A variety of analytical methodologies to investigate gene expression patterns in cells or tissues have been developed. For screening purposes, a large number of target mRNAs have to be interrogated simultaneously. These requirements have been met more or less comprehensively by Differential Display (DD) RT-PCR, Suppression Subtractive Hybridization (SSH), Serial Analysis of Gene Expression (SAGE), and DNA chips. The ultimate goal to cover any gene transcript potentially expressed by a given cell is on the way to be achieved by microbead arrays and by Affymetrix gene chips. Once targets of interest are identified, techniques employing low degrees of multiplexing, such as RNAse protection assays or some bead-based techniques (Luminex) eventually provide extremely fast results on the diagnostic level. With the aid of powerful computer programs, expression profiling technologies have opened intriguing new insights into the complex world of gene regulation. These new techniques have also been applied in drug abuse research recently and some examples of such approaches are described.
已经开发出多种用于研究细胞或组织中基因表达模式的分析方法。出于筛选目的，必须同时对大量靶mRNA进行检测。差异显示（DD）RT-PCR、抑制性消减杂交（SSH）、基因表达序列分析（SAGE）和DNA芯片或多或少地全面满足了这些要求。通过微珠阵列和Affymetrix基因芯片，正在朝着覆盖给定细胞可能表达的任何基因转录本的最终目标迈进。一旦确定了感兴趣的靶标，采用低多重度的技术，如核糖核酸酶保护测定法或一些基于微珠的技术（Luminex）最终可在诊断水平上提供极快的结果。借助强大的计算机程序，表达谱技术为基因调控的复杂世界开启了引人入胜的新见解。这些新技术最近也已应用于药物滥用研究，并描述了一些此类方法的实例。
