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[2001 - 2002年哈瓦那市开展针对埃及伊蚊的强化防治运动期间,从普拉亚市采集的幼虫对杀虫剂的抗性状况及抗性机制]

[Status of resistance to insecticides and resistance mechanisms in larvae from Playa municipality collected during the intensive campaign against Aedes aegypti in Havana City, 2001-2002].

作者信息

Bisset Juan A, Magdalena Rodríguez María, Fernández Ditter, Pérez Omayda

机构信息

Instituto De Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba.

出版信息

Rev Cubana Med Trop. 2004 Jan-Apr;56(1):61-6.

Abstract

The levels of susceptibility and/or resistance to inseciticides in Aedes aegypti larvae collected at the municipality of Playa during the intensive campaign against the mosquito Aedes aegypti, in Havana City, in the present year, were determined. In larvae, it was found a high resistance to the organophosphate insecticides phention and temephos and moderate to fenitrothion. A high resistance to propoxur carbamate was also detected. The larvae proved to be susceptible to clorpirifos, malathion and methylpyrimifos. The results obtained through the use of sinergists indicated that esterases play an important role in the detected resistance to organophosphate insecticides, but not in the resistance to propoxur. However, the monooxigenases take part only in the resistance to fenitothrion. These mechanisms were found at an elevated frequency in the larvae from this municipality. It was confirmed the role played by esterases in the resistance to temephos by polyacrilamide gel inhibition tests.

摘要

测定了今年在哈瓦那市普拉亚市开展的针对埃及伊蚊的强化防治运动期间采集的埃及伊蚊幼虫对杀虫剂的易感性和/或抗性水平。在幼虫中,发现对有机磷杀虫剂倍硫磷和双硫磷具有高抗性,对杀螟硫磷具有中度抗性。还检测到对残杀威氨基甲酸酯具有高抗性。幼虫对毒死蜱、马拉硫磷和甲基嘧啶磷敏感。通过使用增效剂获得的结果表明,酯酶在检测到的对有机磷杀虫剂的抗性中起重要作用,但在对残杀威的抗性中不起作用。然而,单加氧酶仅参与对杀螟硫磷的抗性。在该市的幼虫中发现这些机制的频率较高。通过聚丙烯酰胺凝胶抑制试验证实了酯酶在对双硫磷抗性中的作用。

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