Bisset J A, Rodríguez M M, Ricardo Y, Ranson H, Pérez O, Moya M, Vázquez A
Department of Vector Control, Institute of Tropical Medicine Pedro Kourí, Marianao 13, Havana, Cuba.
Med Vet Entomol. 2011 Sep;25(3):233-9. doi: 10.1111/j.1365-2915.2011.00959.x. Epub 2011 Apr 18.
Aedes aegypti (L.) (Diptera: Culicidae) control programmes in Cuba rely on the application of the organophosphate temephos for larval control. Hence, the monitoring of resistance to this insecticide is an essential component of such programmes. Here, 15 field populations from different municipalities of Havana City were assayed for resistance to temephos. High levels of resistance were detected in all strains and resistance ratios were highly correlated with esterase activity (P = 0.00001). Populations from three municipalities were tested in both 2006 and 2008; resistance and esterase activities both significantly increased during this 2-year period. Synergist studies demonstrated that neither glutathione transferases nor monooxygenases were associated with the increase in resistance to temephos in this period. The duration of the efficacy of commercial formulations of temephos in controlling Ae. aegypti populations in Havana City was reduced by the high level of temephos resistance observed; hence these data are of clear operational significance for the dengue control programme in Cuba. New integrated strategies to avoid further increases in temephos resistance in Cuba are necessary.
古巴的埃及伊蚊控制项目依赖于使用有机磷酸酯类杀虫剂双硫磷来控制幼虫。因此，监测对这种杀虫剂的抗性是此类项目的重要组成部分。在此，对哈瓦那市不同城区的15个野外种群进行了双硫磷抗性检测。在所有品系中均检测到了高水平的抗性，且抗性比率与酯酶活性高度相关（P = 0.00001）。2006年和2008年对来自三个城区的种群进行了检测；在这两年期间，抗性和酯酶活性均显著增加。增效剂研究表明，在此期间，谷胱甘肽转移酶和单加氧酶均与对双硫磷抗性的增加无关。在哈瓦那市，由于观察到的高水平双硫磷抗性，双硫磷商业制剂控制埃及伊蚊种群的药效持续时间缩短；因此，这些数据对古巴的登革热控制项目具有明显的实际意义。有必要采取新的综合策略来避免古巴双硫磷抗性的进一步增加。
