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一种新型细胞周期诱导的MCM家族蛋白MCM9的鉴定。

Identification of a novel cell-cycle-induced MCM family protein MCM9.

作者信息

Yoshida Kenichi

机构信息

Department of Life Sciences, Meiji University, Kanagawa 214-8571, Japan.

出版信息

Biochem Biophys Res Commun. 2005 Jun 3;331(2):669-74. doi: 10.1016/j.bbrc.2005.03.222.

DOI:10.1016/j.bbrc.2005.03.222

PMID:15850810

Abstract

The minichromosome maintenance (MCM) proteins are essential for DNA replication initiation and elongation in eukaryotes. In mammalian cells, MCM2-MCM7 complexes are believed to unwind DNA during chromosomal DNA replication. Here we identified a novel MCM family gene, MCM9, by using bioinformatics. Human, mouse, and rat MCM9 showed approximately 90-91% total-amino acid identity. MCM9 showed 24-31% total-amino acid identity with MCM2-MCM8 protein. Phylogenetic analysis on MCM family members revealed that MCM9 was most closely related to MCM8. Human, mouse, and rat MCM9 gene, consisting of 7, 8, and 7 exons, was mapped to 6q22.1-22.33, 10B3, and 20q11, respectively. We identified transcription factor E2F-binding motifs in the vicinity of the transcription start site among human, mouse, and rat MCM9 gene. MCM9 mRNA was upregulated by transcription factor E2E1 and serum stimulation in NIH3T3 cells.

摘要

微小染色体维持（MCM）蛋白对于真核生物中DNA复制的起始和延伸至关重要。在哺乳动物细胞中，MCM2 - MCM7复合物被认为在染色体DNA复制过程中解旋DNA。在此，我们通过生物信息学方法鉴定了一个新的MCM家族基因MCM9。人、小鼠和大鼠的MCM9在总氨基酸水平上显示出约90 - 91%的同一性。MCM9与MCM2 - MCM8蛋白在总氨基酸水平上具有24 - 31%的同一性。对MCM家族成员的系统发育分析表明，MCM9与MCM8关系最为密切。人、小鼠和大鼠的MCM9基因分别由7个、8个和7个外显子组成，分别定位于6q22.1 - 22.33、10B3和20q11。我们在人、小鼠和大鼠MCM9基因转录起始位点附近鉴定到了转录因子E2F结合基序。在NIH3T3细胞中，MCM9 mRNA受转录因子E2E1和血清刺激而上调。