Yoshida Kenichi
Department of Life Sciences, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
Oncol Rep. 2005 Aug;14(2):531-5.
Members of the F-box protein family are characterized by an approximately 40-amino-acid F-box domain. SCF complexes, formed by Skp1, Cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with Skp1 through the F-box, and with ubiquitination targets. Therefore, F-box proteins are implicated in cell cycle regulation and signal transduction. We characterized the FBXO45 gene, a recently identified F-box protein, as an estrogen-induced gene. By using bioinformatics, human and mouse FBXO45 (286 aa) showed 100% total-amino-acid identity. Human and mouse FBXO45 genes consisting of 3 exons, and the vicinity of the transcriptional start site had several estrogen receptor binding consensus sequences. Finally, human FBXO45 mRNA was rapidly elevated by 17beta-estradiol in MCF-7 cells.
F-box蛋白家族成员的特征是具有一个约40个氨基酸的F-box结构域。由Skp1、Cullin和F-box蛋白形成的SCF复合物作为蛋白质泛素连接酶发挥作用。F-box蛋白通过F-box与Skp1相互作用，并与泛素化靶标相互作用。因此，F-box蛋白参与细胞周期调控和信号转导。我们将最近鉴定出的F-box蛋白FBXO45基因表征为雌激素诱导基因。通过生物信息学分析，人和小鼠的FBXO45（286个氨基酸）的总氨基酸同一性为100%。人和小鼠的FBXO45基因由3个外显子组成，转录起始位点附近有几个雌激素受体结合共有序列。最后，在MCF-7细胞中，17β-雌二醇可使人类FBXO45 mRNA迅速升高。
