人、猴和兔死后眼球晶状体囊膜厚度的非接触光学测量。

Noncontact optical measurement of lens capsule thickness in human, monkey, and rabbit postmortem eyes.

作者信息

Ziebarth Noël M, Manns Fabrice, Uhlhorn Stephen R, Venkatraman Anna S, Parel Jean-Marie

机构信息

Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Miami, FL 33136, USA.

出版信息

Invest Ophthalmol Vis Sci. 2005 May;46(5):1690-7. doi: 10.1167/iovs.05-0039.

DOI:10.1167/iovs.05-0039

PMID:15851570

Abstract

PURPOSE

To measure interspecies thickness differences in the central anterior and posterior capsules of postmortem crystalline lenses, by a technique that maintains the anatomic integrity of the lens.

METHODS

Central capsule thickness was measured with a custom-built, noncontact optical system, using a focus detection technique. Anterior and posterior lens capsule thickness measurements were performed on 22 human, 29 monkey, and 34 New Zealand White rabbit intact postmortem lenses in situ. Eyes were prepared for optical measurements by bonding a PMMA ring to the sclera in the region of the ciliary body after the conjunctiva, adipose, and muscle tissues were removed. The posterior pole was removed by making a circumferential incision through the sclera approximately 7 mm posterior to the limbus. Excess vitreous was removed to expose the posterior capsule surface, and the eye assembly was placed on a Teflon slide. The cornea and iris were sectioned to expose the anterior capsule surface. After the experiments, the lenses were excised, placed in 10% buffered formalin, and prepared for histology. Lens capsule thickness was measured from the histologic slides and compared to the optical

RESULTS

results. Central anterior lens capsule thickness was 8.2 +/- 5.5 (human), 7.5 +/- 4.4 (monkey), and 10.7 +/- 4.2 (rabbit) microm optically and 12.4 +/- 2.5 (human), 10.7 +/- 3.7 (monkey), and 10.4 +/- 2.0 (rabbit) microm histologically. Central posterior capsule thickness was 6.3 +/- 2.2 (human), 5.9 +/- 1.7 (monkey), and 7.8 +/- 2.3 (rabbit) microm optically and 4.1 +/- 1.5 (human), 3.5 +/- 1.6 (monkey), and 4.7 +/- 2.5 (rabbit) microm histologically.

CONCLUSIONS

The central anterior and posterior lens capsule thicknesses do not appear to vary considerably among human, rabbit, and monkey eyes. There were significant differences between optical in situ measurements and histology, which indicates that histologic preparation may affect lens capsule thickness.

摘要

目的

通过一种保持晶状体解剖完整性的技术，测量死后晶状体中央前囊膜和后囊膜的种间厚度差异。

方法

使用定制的非接触光学系统，采用焦点检测技术测量中央囊膜厚度。对22个人类、29只猴子和34只新西兰白兔的完整死后晶状体原位进行前囊膜和后囊膜厚度测量。在去除结膜、脂肪和肌肉组织后，通过在睫状体区域将聚甲基丙烯酸甲酯环粘结到巩膜上，为光学测量准备眼睛。通过在角膜缘后方约7毫米处穿过巩膜进行环形切口，去除后极。去除多余的玻璃体以暴露后囊膜表面，并将眼组件放置在聚四氟乙烯载玻片上。切开角膜和虹膜以暴露前囊膜表面。实验结束后，取出晶状体，放入10%缓冲福尔马林中，准备进行组织学检查。从组织学切片测量晶状体囊膜厚度，并与光学测量结果进行比较。

结果

中央前囊膜厚度光学测量值分别为：人类8.2±5.5微米、猴子7.5±4.4微米、兔子10.7±4.2微米；组织学测量值分别为：人类12.4±2.5微米、猴子10.7±3.7微米、兔子10.4±2.0微米。中央后囊膜厚度光学测量值分别为：人类6.3±2.2微米、猴子5.9±1.7微米、兔子7.8±2.3微米；组织学测量值分别为：人类4.1±1.5微米、猴子3.5±1.6微米、兔子4.7±2.5微米。