Selvam R, Radha Shanmugasundaram K
Enzyme. 1979;24(4):255-60. doi: 10.1159/000458667.
Ca2+-ATPase activity increased five- to six fold when the cells were subjected to growth at 37 degrees C in protein hydrolysate-supplemented media as compared to that of the cells grown in minimal media. One major isoenzyme and one minor isoenzyme were present in minimal-medium-grown cells while two major isoenzymes were present in the cells grown in protein-supplemented media. When the cells were subjected to heat stress (43 degrees C), they exhibited significantly decreased activity as compared to 37 degrees C grown cells. However, all the cultures subjected to growth at 43 degrees C showed two isoenzymes independent of growth medium.
与在基础培养基中生长的细胞相比，当细胞在补充了蛋白水解物的培养基中于37℃培养时，Ca2+-ATP酶活性增加了五到六倍。在基础培养基中生长的细胞中存在一种主要同工酶和一种次要同工酶，而在补充了蛋白质的培养基中生长的细胞中存在两种主要同工酶。当细胞受到热应激（43℃）时，与在37℃生长的细胞相比，它们的活性显著降低。然而，所有在43℃培养的培养物都显示出两种同工酶，与生长培养基无关。
