Zhao Chang-Rong, Liu Bao-Sheng, Zhang Hong-Yi
College of Chemistry and Environment Science, Hebei University, Baoding 071002, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jan;25(1):92-4.
A new method for the determination of albumin in human serum and mouse serum has been developed by spectrophotometry coupled with acid brown SR(ASR) as probe molecule. The maximum absorption wavelength of ASR was at 445 nm, while the maximum absorption wavelength of their product was at 610 nm. However, the reaction of ASR with albumin such as BSA or HSA was so strong that parts of their product were undissoluble in water. The addition of gum water into the system effectively eliminated the deposition. Under optimum reaction conditions, the ranges of working lines for BSA and HSA were 0-91.0 mg x L(-1) and 0-95.2 mg x L(-1), respectively. The detection limits were 5.72 mg x L(-1) for BSA and 5.15 mg x L(-1) for HSA. The relative standard derivation and the recovery of the method for the determination of total proteins in 6 human serum samples were 1.8%-4.4% and 93.6% - 109.1%, respectively. The proposed method has been employed in the assay of protein of human serum and mouse serum. The results of this work were in agreement with those obtained by Biuret method.
已开发出一种以酸性棕SR（ASR）为探针分子，采用分光光度法测定人血清和小鼠血清中白蛋白的新方法。ASR的最大吸收波长为445nm，而其产物的最大吸收波长为610nm。然而，ASR与牛血清白蛋白（BSA）或人血清白蛋白（HSA）等白蛋白的反应非常强烈，以至于它们的部分产物不溶于水。向体系中加入胶水有效地消除了沉淀。在最佳反应条件下，BSA和HSA的工作曲线范围分别为0 - 91.0mg·L⁻¹和0 - 95.2mg·L⁻¹。BSA的检测限为5.72mg·L⁻¹，HSA的检测限为5.15mg·L⁻¹。该方法测定6份人血清样本中总蛋白的相对标准偏差和回收率分别为1.8% - 4.4%和93.6% - 109.1%。所提出的方法已用于人血清和小鼠血清蛋白质的测定。这项工作的结果与用双缩脲法获得的结果一致。
