Péter Antal, Péter Mária, Ilisz István, Fülöp Ferenc
Department of Inorganic and Analytical Chemistry, University of Szeged, H-6720 Szeged, Dóm tér 7, Hungary.
Biomed Chromatogr. 2005 Jul;19(6):459-65. doi: 10.1002/bmc.512.
The enantioseparability of 1- or 3-methyl-substituted tetrahydroisoquinolines was investigated by direct and indirect high-performance liquid chromatography. Alpha- and beta-cyclodextrin-, macrocyclic glycopeptide- and cellulose-based chiral columns and isothiocyanate- and Sanger-type chiral derivatizing agents (CDAs) were applied to attain satisfactory enantioseparation. Of the chiral columns, beta-cyclodextrin-, vancomycin- and teicoplanin-containing macrocyclic glycopeptide-based columns appeared to be most suitable; of the CDAs, (1S,2R)-1-acetoxy-1-phenyl-2-propyl isothiocyanate was most favorable. The differences between the selectivities of the columns and CDAs were compared and optimized to yield the best resolution.
通过直接和间接高效液相色谱法研究了1-或3-甲基取代的四氢异喹啉的对映体可分离性。应用α-和β-环糊精、大环糖肽和纤维素基手性柱以及异硫氰酸酯和桑格型手性衍生化试剂（CDA）以实现令人满意的对映体分离。在手性柱中，基于β-环糊精、含万古霉素和替考拉宁的大环糖肽的柱似乎最合适；在CDA中，（1S，2R）-1-乙酰氧基-1-苯基-2-丙基异硫氰酸酯最有利。比较并优化了柱和CDA选择性之间的差异以获得最佳分离度。
