Fu Xiaoyan, Fukuzawa Mai, Tabata Jun, Tatsuki Sadahiro, Ishikawa Yukio
Laboratory of Applied Entomology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Insect Biochem Mol Biol. 2005 Jun;35(6):621-6. doi: 10.1016/j.ibmb.2005.02.003. Epub 2005 Mar 17.
In order to clarify the biochemical basis to the divergence of sex pheromones in the genus Ostrinia (Lepidoptera: Crambidae), the pheromone biosynthetic pathway in O. zaguliaevi, a close relative of the European corn borer O. nubilalis, was investigated. Deuterium-labeled hexadecanoic or tetradecanoic acids were topically applied to the surface of the pheromone gland, and the incorporation of the label into pheromone components and their putative precursors was determined. It was suggested that the two components shared by O. zaguliaevi and O. nubilalis, (E)-11- and (Z)-11-tetradecenyl acetates, are biosynthesized from hexadecanoic acid through one round of chain shortening, Delta11 desaturation, reduction, and acetylation. An additional component specifically found in O. zaguliaevi, (Z)-9-tetradecenyl acetate, is likely to be produced by delta11 desaturation of hexadecanoic acid, one round of chain shortening, reduction, and acetylation. Non-production of (Z)-9-tetradecenyl acetate in O. nubilalis was suggested to be due to the blockage of chain shortening from (Z)-11-hexadecenoate to (Z)-9-tetradecenoate.
为了阐明欧洲玉米螟属(鳞翅目:草螟科)中性信息素差异的生化基础,对欧洲玉米螟近亲扎古利亚耶夫欧洲玉米螟的性信息素生物合成途径进行了研究。将氘标记的十六烷酸或十四烷酸局部施用于性信息素腺体表面,并测定标记物在性信息素成分及其假定前体中的掺入情况。结果表明,扎古利亚耶夫欧洲玉米螟和欧洲玉米螟共有的两种成分,即(E)-11-和(Z)-11-十四碳烯基乙酸酯,是由十六烷酸通过一轮链缩短、Δ11去饱和、还原和乙酰化生物合成的。扎古利亚耶夫欧洲玉米螟中特有的另一种成分,即(Z)-9-十四碳烯基乙酸酯,可能是由十六烷酸的Δ11去饱和、一轮链缩短、还原和乙酰化产生的。欧洲玉米螟中不产生(Z)-9-十四碳烯基乙酸酯被认为是由于从(Z)-11-十六碳烯酸到(Z)-9-十四碳烯酸的链缩短受阻。