Eltahlawy H S, Buckner J S, Foster S P
Department of Entomology, North Dakota State University, Fargo, ND 58105-5346, USA.
Arch Insect Biochem Physiol. 2007 May;65(1):29-38. doi: 10.1002/arch.20175.
The regulation of pheromone biosynthesis by the neuropeptide PBAN in the Z strain of the European corn borer, Ostrinia nubilalis, was investigated using labeled intermediates. Injection of radiolabeled acetate showed PBAN did not influence the de novo synthesis of saturated fatty acids in the gland. When deuterium-labeled myristic acid was topically applied to the gland, females injected with PBAN produced more labeled pheromone than did control females, indicating that PBAN controls one of the later steps of pheromone biosynthesis. Although more myristic acid was Delta11-desaturated in the gland in the presence of PBAN, this was counterbalanced by less Delta11-desaturation of palmitic acid, indicating that desaturase activity did not change overall. This change in flux of myristic acid through to pheromone was shown to be caused by increased reduction of fatty acid pheromone precursors occurring in the presence of PBAN.
利用标记中间体对欧洲玉米螟Z菌株中神经肽PBAN对性信息素生物合成的调控进行了研究。注射放射性标记的乙酸盐表明，PBAN不影响腺体中饱和脂肪酸的从头合成。当将氘标记的肉豆蔻酸局部施用于腺体时，注射PBAN的雌性产生的标记性信息素比对照雌性更多，这表明PBAN控制着性信息素生物合成的后期步骤之一。尽管在PBAN存在下腺体中更多的肉豆蔻酸发生了Δ11-去饱和，但这被棕榈酸较少的Δ11-去饱和所抵消，表明去饱和酶活性总体上没有变化。肉豆蔻酸通向性信息素的通量变化表明，这是由PBAN存在时脂肪酸性信息素前体的还原增加所致。
Zotero 插件