脂肪酶和酯酶的高通量检测方法。

High-throughput assays for lipases and esterases.

作者信息

Schmidt Marlen, Bornscheuer Uwe T

机构信息

Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr. 16, D-17487 Greifswald, Germany.

出版信息

Biomol Eng. 2005 Jun;22(1-3):51-6. doi: 10.1016/j.bioeng.2004.09.004.

DOI:10.1016/j.bioeng.2004.09.004

PMID:15857783

Abstract

In the past few years a considerable number of high-throughput screening (HTS) systems have been developed, especially for lipases and esterases. In this review, a range of HTS methods for the directed evolution of these hydrolases are covered. This includes spectrophotometric and fluorimetric formats as well as other approaches to allow for fast, efficient and reliable identification of desired enzyme variants within large mutant libraries. In addition, methods for library creation and application of lipases and esterases are briefly covered.

摘要

在过去几年中，已经开发出了相当数量的高通量筛选（HTS）系统，特别是针对脂肪酶和酯酶的系统。在本综述中，涵盖了一系列用于这些水解酶定向进化的高通量筛选方法。这包括分光光度法和荧光法形式以及其他方法，以便在大型突变文库中快速、高效且可靠地鉴定所需的酶变体。此外，还简要介绍了脂肪酶和酯酶文库构建及应用的方法。