Kalir Tamara, Rahaman Jamal, Hagopian George, Demopoulos Rita, Cohen Carmel, Burstein David E
Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Arch Pathol Lab Med. 2005 May;129(5):651-4. doi: 10.5858/2005-129-0651-IDOGTG.
Enhanced expression of GLUT1, a facilitative glucose transporter found on red blood cells, blood-brain barrier, and perineurium, has been described in a large spectrum of epithelial malignancies.
We present an immunohistochemical survey of GLUT1 expression in benign and malignant fallopian tube epithelia, and compare serous carcinomas of the fallopian tube and ovary.
One hundred two routinely fixed and processed archival specimens (36 benign fallopian tubes, 29 primary tubal adenocarcinomas, and 37 primary ovarian adenocarcinomas) were immunostained with rabbit anti-GLUT1 and developed with streptavidin-biotin/diaminobenzidine. Only distinct membrane staining was scored positively (1+ to 3+).
Benign tubes (n = 36) were either negatively stained (58.3%) or displayed rare weak staining (0.5+ to 1+, rarely 2+; 41.7%); of the latter, 4 specimens showed chronic salpingitis, and 6 showed hyperplasia (epithelial tufting and stratification). A case of florid hyperplasia with atypia in a BRCA1-positive patient was GLUT1 negative. Twenty-three (79.3%) of 29 tubal carcinomas were positively stained. Staining ranged from focal/scattered foci (n = 15) to multifocal/extensive (n = 8). Of the 6 nonstaining tubal carcinomas, 3 were undifferentiated. Nineteen tubal carcinoma sections showed residual benign epithelium, which was consistently nonstaining. Very frequently, GLUT1 staining intensified in cells furthest from stroma/ stromal capillaries and/or bordering necrotic zones. On average, GLUT1 staining in primary fallopian tube cancers was less extensive than in primary ovarian adenocarcinomas.
GLUT1 immunostaining of fallopian tube adenocarcinomas was substantially stronger and more extensive than staining of benign tubal epithelium, consistent with previously described findings in carcinomas versus benign tissues from many primary sites. The frequent localization of GLUT1 positivity to regions most distal from stroma/stromal capillaries is consistent with known activation of GLUT1 expression by hypoxia-sensing cellular pathways and may constitute a survival advantage under hypoxic conditions present in malignancy. The difference in extent of GLUT1 staining between primary tubal and primary ovarian serous adenocarcinomas is discussed.
葡萄糖转运蛋白1（GLUT1）是一种易化型葡萄糖转运体，在红细胞、血脑屏障和神经束膜中表达增强，在多种上皮性恶性肿瘤中也有相关报道。
我们对良性和恶性输卵管上皮中的GLUT1表达进行了免疫组化研究，并比较了输卵管和卵巢的浆液性癌。
102例常规固定和处理的存档标本（36例良性输卵管、29例原发性输卵管腺癌和37例原发性卵巢腺癌）用兔抗GLUT1进行免疫染色，并用链霉亲和素-生物素/二氨基联苯胺显色。仅对明显的膜染色进行阳性评分（1+至3+）。
良性输卵管（n = 36）要么呈阴性染色（58.3%），要么显示罕见的弱阳性染色（0.5+至1+，很少为2+；41.7%）；后者中，4例标本显示慢性输卵管炎，6例显示增生（上皮簇状和分层）。1例BRCA1阳性患者的伴有异型性的旺炽性增生病例GLUT1呈阴性。29例输卵管癌中有23例（79.3%）呈阳性染色。染色范围从局灶/散在病灶（n = 15）到多灶/广泛病灶（n = 8）。在6例未染色的输卵管癌中，3例为未分化癌。19例输卵管癌切片显示残留的良性上皮，其始终未染色。非常常见的是，GLUT1染色在距离基质/基质毛细血管最远和/或靠近坏死区的细胞中增强。原发性输卵管癌中GLUT1染色的范围平均比原发性卵巢腺癌小。
输卵管腺癌的GLUT1免疫染色比良性输卵管上皮的染色明显更强且更广泛，这与先前在许多原发性部位的癌组织与良性组织中的发现一致。GLUT1阳性频繁定位于距离基质/基质毛细血管最远的区域，这与已知的缺氧感应细胞途径激活GLUT1表达一致，并且可能在恶性肿瘤中存在的缺氧条件下构成生存优势。讨论了原发性输卵管和原发性卵巢浆液性腺癌之间GLUT1染色范围的差异。
