Zhang Zhi-Hong, Qu Wei-Dong, Zhu Hui-Gang
School of Public Health, Fudan University, Shanghai 200032, China.
Wei Sheng Yan Jiu. 2005 Jan;34(1):40-2.
To explore the regulative action of IEGs when the nicotinic acetylcholine receptors (nAChRs) of PC12 cells were activated by anatoxin-a.
Using the reverse transcription polymerase chain reaction (RT-PCR) method, the mRNA gene expression of c-fos, c-jun, NGFI-A and NGFI-B were measured while PC12 cells were activated by anatoxin-a.
As 10(-9), 10(-8) and 10(-7) mol/L anatoxin-a activated PC12 cells for an hour, or 10(-7) mol/ L anatoxin-a activated PC12 cells for 30 min, 60 min, 120 min, the intracellular gene expression of c-fos and NGFI-A increased significantly than the control group (P < 0.05), it was 2-6 times than the control group. And the gene expression of c-fos presented dose-response and time-effect relation. However, under the same condition, the gene expression of c-jun and NGFI-B did not show any remarkable changes.
c-fos and NGFI-A might be involved to modulation the action of anatoxin-a activating the nAChRs of PC12 cells.
探讨当PC12细胞的烟碱型乙酰胆碱受体（nAChRs）被类毒素-a激活时即刻早期基因（IEGs）的调节作用。
采用逆转录聚合酶链反应（RT-PCR）法，检测类毒素-a激活PC12细胞时c-fos、c-jun、NGFI-A和NGFI-B的mRNA基因表达。
当10^(-9)、10^(-8)和10^(-7)mol/L类毒素-a激活PC12细胞1小时，或10^(-7)mol/L类毒素-a激活PC12细胞30分钟、60分钟、120分钟时，c-fos和NGFI-A的细胞内基因表达比对照组显著增加（P<0.05），是对照组的2至6倍。且c-fos的基因表达呈现剂量反应和时间效应关系。然而，在相同条件下，c-jun和NGFI-B的基因表达未显示任何显著变化。
c-fos和NGFI-A可能参与调节类毒素-a激活PC12细胞nAChRs的作用。
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