中国仓鼠卵巢（CHO）细胞在离子通道研究中的应用。

The use of Chinese hamster ovary (CHO) cells in the study of ion channels.

作者信息

Gamper Nikita, Stockand James D, Shapiro Mark S

机构信息

Department of Physiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio TX 78229, USA.

出版信息

J Pharmacol Toxicol Methods. 2005 May-Jun;51(3):177-85. doi: 10.1016/j.vascn.2004.08.008. Epub 2005 Mar 23.

DOI:10.1016/j.vascn.2004.08.008

PMID:15862463

Abstract

The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).

摘要

上皮样中国仓鼠卵巢（CHO）细胞系由T.T.帕克于1957年建立。从那时起，CHO细胞已成为工业和科学领域广泛使用的哺乳动物表达系统。本文讨论了CHO细胞生理学的不同特征以及将这些细胞用于离子通道研究的具体方面；所讨论的特征包括CHO细胞的培养和转染、从它们进行电生理记录的细节以及离子通道生理学和药理学研究的应用。本文讨论的在CHO细胞中成功重建哺乳动物离子通道的例子包括毒蕈碱型乙酰胆碱受体对KCNQ通道调节的重建以及上皮钠通道（ENaC）对氨氯地平敏感性的研究。