Ahluwalia A, De Rossi D, Ristori C, Schirone A, Serra G
Centro E. Piaggio, Pisa, Italy.
Biosens Bioelectron. 1992;7(3):207-14. doi: 10.1016/0956-5663(92)87017-j.
This paper presents the results of a study of a number of antibody immobilization techniques for application to optical immunosensors. In particular, well-known methods such as covalent binding and physical adsorption have been extended to the Langmiur-Blodgett method in an attempt to improve the density and possibly the uniformity of orientation of monoclonal antibodies on an optical surface. The surface density of active immobilized antibodies was determined from enzyme immunoassay and their thickness and refractive index were deduced from ellipsometry. It is shown that, although high surface densities (500 ng/cm2) of antibody can be obtained, the major obstacle to the detection of low concentrations of antigens or haptens is the non-specific binding of foreign molecules to the sensing surface.
本文介绍了一系列用于光学免疫传感器的抗体固定技术的研究结果。特别是，诸如共价结合和物理吸附等知名方法已扩展到朗缪尔-布洛杰特方法，试图提高单克隆抗体在光学表面上的密度以及可能的取向均匀性。通过酶免疫测定法测定活性固定抗体的表面密度，并通过椭圆偏振法推导其厚度和折射率。结果表明，尽管可以获得高表面密度（500 ng/cm2）的抗体，但检测低浓度抗原或半抗的主要障碍是外来分子与传感表面的非特异性结合。
