Gaer J A, Wharton J, Gordon L, Swift R I, Munsch C, Inglis G C, Polak J M, Taylor K M
Department of Cardiothoracic Surgery, Hammersmith Hospital, London, UK.
Eur J Cardiothorac Surg. 1992;6(4):201-8. doi: 10.1016/1010-7940(92)90217-l.
Twenty-six calves were subjected to a technique of cryoablation in order to establish an animal model of complete cardiac denervation. All 26 survived the procedure, and 20 were alive to be re-evaluated 2-4 weeks later. Mean heart rate in the denervated animals rose from 77 +/- 7.8 beats/min to 102 +/- 16.4 (P less than 0.01). Cryoablation abolished the heart rate responses to electrical stimulation of the vagus nerve and thoracic sympathetic trunk. The reduction in myocardial noradrenaline concentrations averaged 99% in the right atrium, 90% in the left atrium, 85% in the right ventricle and 90% in the left ventricle, when compared with tissue obtained from control animals. Cryoablation is a relatively simple means of accomplishing complete functional cardiac denervation in the calf. On the basis of the observed change in heart rate, the calf model appears to be more comparable with human heart transplant recipients than the dog.
26头小牛接受了冷冻消融技术，以建立完全心脏去神经支配的动物模型。所有26头小牛在手术中存活下来，20头存活至2 - 4周后接受重新评估。去神经支配动物的平均心率从77±7.8次/分钟升至102±16.4次/分钟（P<0.01）。冷冻消融消除了对迷走神经和胸交感干电刺激的心率反应。与对照动物的组织相比，右心房心肌去甲肾上腺素浓度平均降低99%，左心房降低90%，右心室降低85%，左心室降低90%。冷冻消融是在小牛中实现完全功能性心脏去神经支配的相对简单的方法。基于观察到的心率变化，小牛模型似乎比狗模型更类似于人类心脏移植受者。
