评估锌对蛋白质缺乏大鼠血清和肝脏中肝脏标志物酶及元素谱的疗效的时间依赖性研究。

Sidhu Pardeep, Garg M L, Dhawan D K

Department of Biophysics, Panjab University, Chandigarh UT 160014, India.

Biometals. 2005 Feb;18(1):97-106. doi: 10.1007/s10534-004-1960-y.

This study was designed to determine the time dependent protective effects of zinc sulfate on the serum and liver marker enzymes along with elemental profile in protein deficient Sprague Dawley (S.D.) female rats. Zinc sulfate in the dose of 227 mg/l in drinking water was administered to normal control as well as protein deficient rats for a total duration of 8 weeks. The effects of different treatments were studied on enzymes like alkaline phosphatase (ALP), aspartate aminotransferases (AST) and alanine aminotransferases (ALT) in rat serum at different time intervals of 1, 2, 4 and 8 weeks and in the rat liver at the end of study. The status of different essential elements in liver was also studied. The serum ALP activity got significantly depressed when estimated at the intervals of 4 and 8 weeks. Activity of serum ALT was significantly increased after 4 weeks interval in protein deficient rats and the increasing trend continued upto 8 weeks of protein deficiency. On the other hand, activity of AST showed a significant increase just after 2 weeks and activity continued to be increased up to 8 weeks. Moreover activities of all the hepato marker enzymes showed a significant increase in liver of protein deficient rats. Interestingly, supplementation of Zn to protein deficient rats helped in regulating the altered activities of ALP, AST and ALT both in serum and liver. However, zinc treatment alone to normal rats did not indicate any significant change in the activities of all the enzymes in liver as well serum except at the interval of 2 weeks where a marginal increase in the activity of AST was seen. It has also been observed that concentrations of zinc, copper, iron and selenium were found to be decreased significantly in protein deficient animals. However, the levels of these elements came back to within normal limits when zinc was administered to protein deficient rats.

本研究旨在确定硫酸锌对蛋白质缺乏的斯普拉格-道利（S.D.）雌性大鼠血清和肝脏标记酶以及元素分布随时间变化的保护作用。将饮用水中剂量为227 mg/l的硫酸锌给予正常对照组以及蛋白质缺乏的大鼠，持续8周。在1、2、4和8周的不同时间间隔研究不同处理对大鼠血清中碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）等酶的影响，并在研究结束时研究大鼠肝脏中的这些酶。还研究了肝脏中不同必需元素的状态。在4周和8周间隔时估计血清ALP活性显著降低。蛋白质缺乏大鼠在4周间隔后血清ALT活性显著增加，且在蛋白质缺乏8周时这种增加趋势持续。另一方面，AST活性在2周后立即显著增加，并持续增加至8周。此外，所有肝脏标记酶的活性在蛋白质缺乏大鼠的肝脏中均显著增加。有趣的是，向蛋白质缺乏大鼠补充锌有助于调节血清和肝脏中ALP、AST和ALT的改变活性。然而，单独给正常大鼠补锌除了在2周间隔时AST活性有轻微增加外，肝脏和血清中所有酶的活性均未显示任何显著变化。还观察到，蛋白质缺乏动物体内锌、铜、铁和硒的浓度显著降低。然而，当给蛋白质缺乏大鼠补锌时，这些元素的水平恢复到正常范围内。