Arief Erry Mochamad, Mohamed Zeehaida, Idris Fauziah Mohamad
School of Dental Sciences, Universiti Sains Malaysia, Kota Bharu, Kelantan, Malaysia.
Cleft Palate Craniofac J. 2005 May;42(3):277-9. doi: 10.1597/04-083r.1.
To determine the effect of surgery on types and colony count of Streptococcus and Staphylococcus species in cleft lip and palate (CLP) patients.
Saliva samples were collected after the morning meal by placing a sterile cotton swab in the vestibule of the oral cavity from cleft lip and palate patients immediately preoperative and 12 weeks postoperative. Normal children were examined as a control group. Samples were cultured; Staphylococcus and Streptococcus isolates were identified and quantified.
Fifteen cleft lip and palate patients and 22 normal children, aged 3 to 39 months were examined.
Streptococcus mitis biovar 1, Streptococcus salivarius and Streptococcus oralis of the viridans group of streptococci were the most commonly found in normal children, as well as in cleft lip and palate children. In the cleft lip and palate group, mean streptococcal count was 32.41 (29.80) and 46.46 (42.80) in the pre- and postoperative periods, respectively; in the normal group, the count was 20.93 (27.93) and 49.92 (34.72) at 0 week and 12 weeks, respectively. Staphylococcus aureus was the most common Staphylococcus species found in CLP patients, representing 47.4% postoperatively. In the cleft lip and palate children, mean staphylococcal count was 5.34 (8.13) and 0.56 (0.92) in the pre- and postoperative periods, respectively; in normal children, the count was 0.82 (1.98) and 0.60 (2.55) at 0 and 12 weeks, respectively. The differences were statistically significant only for the staphylococcal count between pre- and postoperative periods in children with cleft lip and palate as tested by analysis of variance (p < .05).
Cleft lip and palate patients had more colonization by S. aureus compared with normal children, and the colony count decreased significantly following surgical repair of the cleft lip and palate.
确定手术对唇腭裂（CLP）患者口腔中链球菌和葡萄球菌种类及菌落计数的影响。
在早餐后，通过将无菌棉签置于唇腭裂患者口腔前庭来采集唾液样本，分别于术前即刻和术后12周进行。选取正常儿童作为对照组进行检查。对样本进行培养；鉴定并定量葡萄球菌和链球菌分离株。
检查了15例年龄在3至39个月的唇腭裂患者和22名正常儿童。
在正常儿童以及唇腭裂儿童中，最常见的是甲型溶血性链球菌1型、唾液链球菌和口腔链球菌等草绿色链球菌群。在唇腭裂组中，术前和术后链球菌平均菌落计数分别为32.41（29.80）和46.46（42.80）；在正常组中，0周和12周时的计数分别为20.93（27.93）和49.92（34.72）。金黄色葡萄球菌是唇腭裂患者中最常见的葡萄球菌种类，术后占47.4%。在唇腭裂儿童中，术前和术后葡萄球菌平均菌落计数分别为5.34（8.13）和0.56（0.92）；在正常儿童中，0周和12周时的计数分别为0.82（1.98）和0.60（2.55）。经方差分析，仅唇腭裂儿童术前和术后葡萄球菌计数的差异具有统计学意义（p <.05）。
与正常儿童相比，唇腭裂患者口腔中金黄色葡萄球菌的定植更多，唇腭裂手术修复后菌落计数显著下降。
