Dietrich G, Algiman M, Sultan Y, Nydegger U E, Kazatchkine M D
INSERM U28, Hôpital Broussais, Paris, France.
Blood. 1992 Jun 1;79(11):2946-51.
Therapeutic preparations of polyspecific IgG obtained from plasma pools of a large number of normal donors (IVIg) express anti-idiotypic activity against a wide spectrum of natural and disease-associated autoantibodies. The present study investigated the origin of anti-idiotypic activity against autoantibodies to factor VIII. The neutralizing activity of pools of IgG against patients' anti-factor VIII autoantibodies was not influenced by the presence of individuals with natural anti-factor VIII antibodies among donors contributing to the pool. A higher frequency of neutralizing antibodies against anti-factor VIII autoantibodies was found in aged donors as compared with young adults and in pools of IgG from multiparous women as compared with IgG from random donors. Pooling IgG from several donors synergistically enhanced the inhibitory activity of the pools. Thus, a neutralizing activity against anti-factor VIII autoantibodies was detected in pools of IgG of as few as two to four donors of whom individually tested IgG did not exhibit inhibitory activity against anti-factor VIII autoantibodies. These observations suggest that aged donors and multiparous women may be privileged sources for the anti-idiotypic activity of IVIg against autoantibodies and emphasize that the expression of anti-idiotypic activity in IVIg results from a synergistic participation of anti-idiotypes from each donor contributing to the pool.
从大量正常供体的血浆库中获得的多特异性IgG治疗制剂（静脉注射免疫球蛋白，IVIg）对多种天然和疾病相关自身抗体具有抗独特型活性。本研究调查了针对因子VIII自身抗体的抗独特型活性的来源。IgG库对患者抗因子VIII自身抗体的中和活性不受献血浆库的供体中存在天然抗因子VIII抗体个体的影响。与年轻成年人相比，老年供体中针对抗因子VIII自身抗体的中和抗体频率更高；与随机供体的IgG相比，多产妇女的IgG库中针对抗因子VIII自身抗体的中和抗体频率更高。将来自多个供体的IgG混合可协同增强混合液的抑制活性。因此，在仅有两到四个供体的IgG库中检测到了针对抗因子VIII自身抗体的中和活性，而单独检测时这些供体的IgG对抗因子VIII自身抗体没有抑制活性。这些观察结果表明，老年供体和多产妇女可能是IVIg针对自身抗体的抗独特型活性的优先来源，并强调IVIg中抗独特型活性的表达是由每个献血浆库的供体的抗独特型协同参与所致。
