一种通过液相色谱/单四极杆质谱法筛查血清中各种镇静催眠药的方法。

A method for screening for various sedative-hypnotics in serum by liquid chromatography/single quadrupole mass spectrometry.

作者信息

Miyaguchi Hajime, Kuwayama Kenji, Tsujikawa Kenji, Kanamori Tatsuyuki, Iwata Yuko T, Inoue Hiroyuki, Kishi Tohru

机构信息

National Research Institute of Police Science, Kashiwa-shi, Chiba, Japan.

出版信息

Forensic Sci Int. 2006 Feb 10;157(1):57-70. doi: 10.1016/j.forsciint.2005.03.011.

DOI:10.1016/j.forsciint.2005.03.011

PMID:15869852

Abstract

A screening method for the detection of sedative-hypnotics in serum is described. The target drugs, which include practically all the sedative-hypnotics distributed in Japan, consisted of 5 barbiturates, 30 benzodiazepine-related drugs and 11 other sedative-hypnotics (i.e., apronalide, bromisovalum, chloral hydrate, triclofos, chlorpromazine, promethazine, diphenhydramine, hydroxyzine, zopiclone, zolpidem and tandospirone). Thirty-nine analytes, selected in terms of the pharmacokinetics of the target drugs, in human serum were screened using a combination of mixed-mode solid-phase extraction and liquid chromatography/electrospray-ionization single-quadrupole mass spectrometry. The detection limits (non-basic analytes, 1-50 ng/ml; basic analytes, 0.1-5 ng/ml) were sufficient to permit the screening of a single therapeutic administration of a target drug.

摘要

本文描述了一种检测血清中镇静催眠药的筛查方法。目标药物包括在日本销售的几乎所有镇静催眠药，由5种巴比妥类药物、30种苯二氮䓬类相关药物和11种其他镇静催眠药（即阿普唑仑、溴米索伐、水合氯醛、三氯福司、氯丙嗪、异丙嗪、苯海拉明、羟嗪、佐匹克隆、唑吡坦和坦度螺酮）组成。根据目标药物的药代动力学，从人血清中选择了39种分析物，采用混合模式固相萃取和液相色谱/电喷雾电离单四极杆质谱联用技术进行筛查。检测限（非碱性分析物为1-50 ng/ml；碱性分析物为0.1-5 ng/ml）足以对目标药物的单次治疗给药进行筛查。