Bando Takayoshi, Sekine Keisuke, Kobayashi Shizuka, Watabe Ayako M, Rump Armin, Tanaka Minoru, Suda Yoshikuni, Kato Shigeaki, Morikawa Yoshihiro, Manabe Toshiya, Miyajima Atsushi
Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Mol Cell Biol. 2005 May;25(10):4166-75. doi: 10.1128/MCB.25.10.4166-4175.2005.
Neuronal leucine-rich repeat proteins (NLRRs) are type I transmembrane proteins and expressed in neuronal tissues, but their function remains unknown. Here, we describe the identification and characterization of a new member of the NLRR family, NLRR4, and its potential role in long-lasting memory. We generated NLRR4-deficient (NLRR4(-/-)) mice and found that they showed impaired memory retention. In hippocampus-dependent learning tasks, NLRR4(-/-) mice were able to learn and maintain the memories for one day but unable to retain the memories for four days after learning. In contrast, in a hippocampus-independent task, NLRR4(-/-) mice were able to retain the memory normally for at least seven days. These results suggest that NLRR4 plays a key role in hippocampus-dependent long-lasting memory.
神经元富含亮氨酸重复序列蛋白（NLRRs）是I型跨膜蛋白，在神经组织中表达，但其功能尚不清楚。在此，我们描述了NLRR家族新成员NLRR4的鉴定和特征，以及它在长期记忆中的潜在作用。我们培育了NLRR4基因缺失（NLRR4(-/-)）小鼠，发现它们的记忆保持受损。在依赖海马体的学习任务中，NLRR4(-/-)小鼠能够学习并将记忆维持一天，但在学习后四天无法保留记忆。相比之下，在不依赖海马体的任务中，NLRR4(-/-)小鼠能够将记忆正常保留至少七天。这些结果表明，NLRR4在依赖海马体的长期记忆中起关键作用。
