Yoshida Kenichi
Department of Life Sciences, Meiji University, Kawasaki, Kanagawa 214-8571, Japan.
Oncol Rep. 2005 Jun;13(6):1133-7.
Kelch-like proteins are implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL (kelch homolog) genes, containing two evolutionary conserved domains--broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, and kelch motif, are human homologs of Drosophila kelch gene. We identified the KLHL15 gene, a novel human homolog of Drosophila kelch, by using bioinformatics. KLHL15 gene, consisting of 4 exons, was located within human genome sequences RP11-47911 (AC079169.32) and RP11-793H5 (AC079376.26). Complete coding sequence of human KLHL15 cDNA was determined by assembling FLJ32736 cDNA (AK057298) and KIAA1677 cDNA (AB051464). The human, chicken, and zebra fish KLHL15 (604 aa) showed 85-93% total-amino acid identity. N-terminal BTB/POZ domain and C-terminal three KELCH motifs were identified within KLHL15 protein by using the Pfam program. Human KLHL15 mRNA was expressed ubiquitously in various tissues. This is the first report on identification and characterization of the KLHL15 gene.
类kelch蛋白通过细胞骨架组织参与胚胎发生和致癌作用。KLHL（kelch同源物）基因包含两个进化保守结构域——广泛复合体、tramtrack、bric-a-brac/痘病毒和锌指（BTB/POZ）结构域以及kelch基序，是果蝇kelch基因的人类同源物。我们通过生物信息学鉴定了KLHL15基因，它是果蝇kelch的一种新型人类同源物。KLHL15基因由4个外显子组成，位于人类基因组序列RP11 - 47911（AC079169.32）和RP11 - 793H5（AC079376.26）内。通过组装FLJ32736 cDNA（AK057298）和KIAA1677 cDNA（AB051464）确定了人类KLHL15 cDNA的完整编码序列。人类、鸡和斑马鱼的KLHL-15（604个氨基酸）显示出85%-93%的总氨基酸同一性。通过使用Pfam程序在KLHL15蛋白中鉴定出了N端BTB/POZ结构域和C端的三个kelch基序。人类KLHL15 mRNA在各种组织中广泛表达。这是关于KLHL15基因鉴定和特征描述的首次报道。
