血管活性肠肽和垂体腺苷酸环化酶激活多肽刺激前列腺癌细胞和前列腺上皮细胞产生白细胞介素-6。
Vasoactive intestinal peptide and pituitary adenylate cyclase activating polypeptide stimulate interleukin-6 production in prostate cancer cells and prostatic epithelial cells.
作者信息
Nagakawa Osamu, Junicho Akira, Akashi Takuya, Koizumi Keiichi, Matsuda Tadashi, Fuse Hideki, Saiki Ikuo
机构信息
Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
出版信息
Oncol Rep. 2005 Jun;13(6):1217-21.
We investigated the effect of the vasoactive intestinal (VIP) and pituitary adenylate cyclase activating peptides (PACAP) on the production of interleukin-6 (IL-6) in normal prostate epithelial and stromal cells and prostate cancer cells. We performed RT-PCR analysis to assess the expression of VIP receptor (VPAC1, VPAC2 and PAC1) mRNA in normal prostate epithelial and stromal cells and prostate cancer cells, and investigated the effect of VIP and PACAP on the production of IL-6. VPAC1, VPAC2 and PAC1 receptor mRNAs were expressed in LNCaP and DU-145/AR prostate cancer cells and PrEC cells (prostate epithelial cells). VIP stimulated the production of IL-6 in DU-145/AR prostate cancer and PrEC cells. PACAP showed a similar effect on IL-6 production in PrEC cells. VIP stimulated IL-6 promoter transcriptional activity in DU-145/AR cells. These results indicate that VIP and PACAP may modulate the IL-6 production of normal prostate epithelial and prostate cancer cells.
我们研究了血管活性肠肽（VIP）和垂体腺苷酸环化酶激活肽（PACAP）对正常前列腺上皮细胞、基质细胞及前列腺癌细胞中白细胞介素-6（IL-6）产生的影响。我们进行了逆转录聚合酶链反应（RT-PCR）分析，以评估VIP受体（VPAC1、VPAC2和PAC1）mRNA在正常前列腺上皮细胞、基质细胞及前列腺癌细胞中的表达，并研究了VIP和PACAP对IL-6产生的影响。VPAC1、VPAC2和PAC1受体mRNA在LNCaP和DU-145/AR前列腺癌细胞以及PrEC细胞（前列腺上皮细胞）中均有表达。VIP刺激了DU-145/AR前列腺癌细胞和PrEC细胞中IL-6的产生。PACAP对PrEC细胞中IL-6的产生表现出类似的作用。VIP刺激了DU-145/AR细胞中IL-6启动子的转录活性。这些结果表明，VIP和PACAP可能调节正常前列腺上皮细胞和前列腺癌细胞中IL-6的产生。
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