Matsuura T, West G A, Klein J S, Ferraris L, Fiocchi C
Department of Gastroenterology and Research Institute, Cleveland Clinic Foundation, Ohio.
Gastroenterology. 1992 Jun;102(6):2006-14. doi: 10.1016/0016-5085(92)90326-t.
Serum levels of soluble interleukin 2 receptor (sIL-2R) have been proposed as a clinical marker of inflammatory bowel disease. The source of sIL-2R in patients with Crohn's disease and ulcerative colitis is unknown, and other soluble receptors have not been investigated. In the present study, sIL-2R and soluble CD8 and CD4 levels were measured in plasma and culture supernatants of peripheral blood and intestinal mucosal mononuclear cells from patients with inflammatory bowel disease, surgical controls, and healthy subjects. Level of plasma sIL-2R was significantly higher in patients with Crohn's disease and ulcerative colitis than in healthy volunteers. Intestinal cells always produced more sIL-2R than peripheral cells. Spontaneous sIL-2R production by mucosal cells was significantly elevated in Crohn's disease but not in ulcerative colitis supernatants compared with levels of surgical controls. Soluble CD8 and CD4 were poor indicators of systemic or mucosal immunity. A positive correlation was found between plasma sIL-2R and spontaneous production by intestinal cells of patients with Crohn's disease and surgical control patients, whereas ulcerative colitis plasma sIL-2R correlated with spontaneous production by peripheral cells. The association of plasma or spontaneous sIL-2R levels with the degree of intestinal inflammation was weak, and there was a wide overlap with control values. Therefore, caution should be used before considering sIL-2R an accurate marker of inflammatory bowel disease activity.
血清可溶性白细胞介素2受体（sIL-2R）水平已被提议作为炎症性肠病的临床标志物。克罗恩病和溃疡性结肠炎患者体内sIL-2R的来源尚不清楚，其他可溶性受体也未得到研究。在本研究中，对炎症性肠病患者、手术对照者及健康受试者外周血和肠黏膜单个核细胞的血浆及培养上清液中的sIL-2R、可溶性CD8和CD4水平进行了检测。克罗恩病和溃疡性结肠炎患者血浆sIL-2R水平显著高于健康志愿者。肠细胞产生的sIL-2R总是多于外周细胞。与手术对照者的水平相比，克罗恩病患者黏膜细胞自发产生的sIL-2R显著升高，但溃疡性结肠炎患者的培养上清液中未出现这种情况。可溶性CD8和CD4并非全身或黏膜免疫的良好指标。在克罗恩病患者和手术对照患者中，血浆sIL-2R与肠细胞自发产生的sIL-2R之间呈正相关，而溃疡性结肠炎患者血浆sIL-2R与外周细胞自发产生的sIL-2R相关。血浆或自发sIL-2R水平与肠道炎症程度之间的关联较弱，且与对照值有很大重叠。因此，在将sIL-2R视为炎症性肠病活动的准确标志物之前应谨慎。
