Rappuoli R, Pizza M, De Magistris M T, Podda A, Bugnoli M, Manetti R, Nencioni L
Sclavo Research Center, Siena, Italy.
Immunobiology. 1992 Feb;184(2-3):230-9. doi: 10.1016/S0171-2985(11)80477-8.
In 1924 Ramon described the inactivation of diphtheria toxin by formaldehyde treatment. This method allowed the introduction of mass vaccination against diphtheria and tetanus and opened the way to the inactivation of viruses by chemical treatment. In this review we describe the use of genetic manipulations for the inactivation of pertussis toxin. The toxin inactivated by this new method is an antigen superior to those obtained by chemical treatment and has been used to develop a new vaccine against whooping cough.
1924年，拉蒙描述了用甲醛处理使白喉毒素失活的方法。这种方法使得大规模接种白喉和破伤风疫苗成为可能，并为通过化学处理使病毒失活开辟了道路。在这篇综述中，我们描述了利用基因操作使百日咳毒素失活的方法。用这种新方法失活的毒素作为一种抗原，优于通过化学处理获得的抗原，已被用于研发一种新型百日咳疫苗。
