Morioka Cíntia Yoko, Saito Seiji, Machado Marcel Cerqueira Cesar, Jukemura José, Bacchella Telesforo, Watanabe Akiharu
Department of Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil.
In Vivo. 2005 May-Jun;19(3):639-41.
We have previously reported on the "return trip" metastases from the liver to the pancreas in a hamster experimental pancreatic cancer model. Because the pancreas is the main metastatic site of liver-implanted pancreatic tumors, our aim was to clarify whether the metastatic sites differ in young and old tumor-bearing animals. HaP-T1, a continuous tissue-cultured cell line, derived from BHP-induced pancreatic adenocarcinoma, was implanted into the liver. The animals were divided into two groups: A) younger than 26 weeks and B) older than 26 weeks. Three animals from each group were sacrificed on Days 35, 42, 49, 56, 63, 70, 77, 84, 91 and 98, to study the metastatic sites. Survival was also studied. After death, necropsy was performed. Resected and necropsied specimens were analyzed histopathologically and by PCR/RFLP analysis to confirm the presence of K-ras point mutation. The success rate of implantation was 100%. Survival was 102.3+/-2.5 days in group A and 95.3+/-1.5 days in group B. Animals of group A, sacrificed weekly until Day 70, showed metastases only to the pancreas ("return trip"), while this phenomenon happened only in animals sacrificed on Day 35 in group B. In group A, on Days 77, 84, 91 and 98, metastases were also found in the kidneys, lymph nodes, ovary and testis. In hamsters of group B, metastases were found in multiple sites such as the pancreas, vas deferens, ovary and testis ("multiple journeys"). All intra-hepatically-implanted tumor and metastatic sites showed the K-ras point mutation. This homologous implantation model may be helpful for further research into the process of metastasis and its relationship with the immunological response.
我们之前曾报道过在仓鼠实验性胰腺癌模型中，肝脏向胰腺的“返程”转移。由于胰腺是肝脏植入性胰腺肿瘤的主要转移部位，我们的目的是阐明在年轻和年老的荷瘤动物中转移部位是否存在差异。将源自BHP诱导的胰腺腺癌的连续组织培养细胞系HaP-T1植入肝脏。动物被分为两组：A组）小于26周龄，B组）大于26周龄。每组三只动物在第35、42、49、56、63、70、77、84、91和98天被处死，以研究转移部位。同时也对生存情况进行了研究。动物死亡后进行尸检。对切除和尸检的标本进行组织病理学分析以及PCR/RFLP分析，以确认K-ras点突变的存在。植入成功率为100%。A组的生存时间为102.3±2.5天，B组为95.3±1.5天。A组每周处死直至第70天的动物仅显示向胰腺转移（“返程”），而这种现象仅在B组第35天处死的动物中出现。在A组中，第77、84、91和98天在肾脏、淋巴结、卵巢和睾丸中也发现了转移。在B组仓鼠中，在胰腺、输精管、卵巢和睾丸等多个部位发现了转移（“多程转移”）。所有肝内植入肿瘤及转移部位均显示K-ras点突变。这种同源植入模型可能有助于进一步研究转移过程及其与免疫反应的关系。
