Kim Sung Hak, Choi Ji Young, Sihn Choon-Ryoul, Suh Eun Jung, Kim Sun Young, Choi Kang Duk, Jeon Iksoo, Han Jae Yong, Kim Tae-Yoon, Kim Sang Hoon
Department of Biology, College of Science, Kyung Hee University, Seoul 130-701, Korea.
Mol Cells. 2005 Apr 30;19(2):185-90.
The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2- dependent pathway.
鸡输卵管是一个动态器官，能产生诸如卵清蛋白等分泌蛋白，其细胞会经历细胞增殖和分化。目前尚无关于鸡输卵管细胞死亡所涉及细胞机制的研究。因此，本研究聚焦于原代输卵管细胞凋亡的研究。由于已知神经酰胺可激活肿瘤细胞中的凋亡，且在输卵管中产生，我们使用外源性神经酰胺类似物诱导细胞死亡。经神经酰胺处理的鸡输卵管细胞活力呈剂量依赖性下降，通过用膜联蛋白V染色检测到凋亡细胞。通过逆转录聚合酶链反应评估凋亡相关基因的表达，发现暴露于神经酰胺后bcl - 2 mRNA减少，而Bcl - x mRNA在处理后12小时增加。此外，半胱天冬酶 - 3在凋亡早期强烈表达，而半胱天冬酶 - 1和 - 9转录本在后期增加。我们得出结论，神经酰胺通过半胱天冬酶和bcl - 2依赖性途径诱导输卵管来源的原代细胞凋亡。
