Yu Hee Chul, Moon Jang Il, Jin Zhe-Wu, Lee Dae Yung, Kim Chan Young, Song Chang Ho, Cho Baik Hwan
Department of Surgery, Chonbuk National University Medical School, 634-18 Keumam-dong, Dukjin-gu, Jeonju, Jeonbuk, 561-180, Korea.
World J Surg. 2005 Jun;29(6):739-43. doi: 10.1007/s00268-005-7728-x.
We investigated the effect of radiofrequency ablation (RFA) of the liver on cell-mediated immunity in rats. Sprague-Dawley rats were divided into five groups: control group that did not have any procedure, a sham-operation group that underwent laparotomy, a lobectomy group that had left lateral lobectomy of the liver, a one-lobe RFA group that had RFA on the left lateral lobe of the liver, and a two-lobe RFA group that had RFA on the left lateral and left median lobe of the liver. Delayed-type hypersensitivity (DTH) was induced by challenging the ears of previously sensitized rats with 2,4-dinitro-1-fluorobenzene. Cell-mediated immunity was evaluated by direct measurement of the ear thickness and quantitative analysis of leukocytic infiltration of the tissue. The increased ear thickness was 42.0%, 69.2%, 61.8%, 46.7%, and 39.8% from the baseline one day after each procedure, and the mean leukocytic infiltration into the ear tissue was 3136.7, 3895.0, 3913.3, 2803.3, and 2316.7/mm(2) seven days after each procedure in the control, sham-operation, lobectomy, one-lobe RFA, and two-lobe RFA groups, respectively. In conclusion, RFA of the liver tissue can partially abolish the augmentation of DTH and leukocytic infiltration those were seen in the hepatic lobectomy and sham-operation groups. The procedure had a negative effect on cell-mediated immunity in rats, and the intensity of RFA showed a reverse correlation with cell-mediated immunity.
我们研究了肝脏射频消融（RFA）对大鼠细胞介导免疫的影响。将Sprague-Dawley大鼠分为五组：未进行任何操作的对照组、接受剖腹手术的假手术组、进行左肝外侧叶切除术的肝叶切除组、对左肝外侧叶进行RFA的单叶RFA组以及对左肝外侧叶和左中叶进行RFA的双叶RFA组。通过用2,4-二硝基-1-氟苯刺激先前致敏大鼠的耳部来诱导迟发型超敏反应（DTH）。通过直接测量耳部厚度和对组织白细胞浸润进行定量分析来评估细胞介导免疫。每次手术后一天，耳部厚度相对于基线的增加分别为42.0%、69.2%、61.8%、46.7%和39.8%；在对照组、假手术组、肝叶切除组、单叶RFA组和双叶RFA组中，每次手术后七天，耳部组织中的平均白细胞浸润分别为3136.7、3895.0、3913.3、2803.3和2316.7/mm²。总之，肝组织RFA可部分消除肝叶切除组和假手术组中出现的DTH增强和白细胞浸润。该操作对大鼠的细胞介导免疫有负面影响，且RFA的强度与细胞介导免疫呈负相关。
