使用敲入方法实现Cre重组酶的L7/Pcp-2特异性表达。

L7/Pcp-2-specific expression of Cre recombinase using knock-in approach.

作者信息

Saito Hiromitsu, Tsumura Hideki, Otake Seiichi, Nishida Akihiro, Furukawa Takahisa, Suzuki Noboru

机构信息

Department of Animal Genomics, Functional Genomics Institute, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.

出版信息

Biochem Biophys Res Commun. 2005 Jun 17;331(4):1216-21. doi: 10.1016/j.bbrc.2005.04.043.

DOI:10.1016/j.bbrc.2005.04.043

PMID:15883005

Abstract

We report a knock-in mouse expressing Cre recombinase from the translational initiation site (ATG) of the endogenous L7/Pcp-2 gene. The resulting Cre expression matches the pattern of L7/Pcp-2 expression that is restricted to cerebellar Purkinje cells and retinal cells. Moreover, the Cre mouse showed no significant behavioral abnormality. Thus, our novel Cre mouse can be used for generation of Purkinje cells and retinal cell-specific gene expression and/or knockout in mouse using the Cre/loxP system.

摘要

我们报道了一种敲入小鼠，其从内源性L7/Pcp-2基因的翻译起始位点（ATG）表达Cre重组酶。所产生的Cre表达与L7/Pcp-2的表达模式相匹配，该模式仅限于小脑浦肯野细胞和视网膜细胞。此外，Cre小鼠没有表现出明显的行为异常。因此，我们的新型Cre小鼠可用于利用Cre/loxP系统在小鼠中产生浦肯野细胞和视网膜细胞特异性基因表达和/或基因敲除。