单纯疱疹病毒1型的蛋白ICP0定位于受感染细胞的核仁。简报。

The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells. Brief report.

作者信息

Morency E, Couté Y, Thomas J, Texier P, Lomonte P

机构信息

Centre de Génétique Moléculaire et Cellulaire, UMR5534-CNRS, Equipe Silencing Viral et Remodelage de la Chromatine Université Claude Bernard Lyon 1, Villeurbanne, France.

出版信息

Arch Virol. 2005 Nov;150(11):2387-95. doi: 10.1007/s00705-005-0546-5. Epub 2005 May 11.

DOI:10.1007/s00705-005-0546-5

PMID:15883654

Abstract

This study describes the nucleolar localization of the viral protein ICP0 of herpes simplex virus type 1. We show that the RING finger domain of ICP0 is essential for ICP0 to localize in nucleoli of transfected and 4 hour-infected cells. ICP0 forms particular intranucleolar domains that do not correspond to any known nucleolar domains. This distribution was confirmed by immunoblots performed on fractionated infected cells. Quantitative RT-PCR experiments indicated that ICP0 did not increase the transcription from the RNA polymerase I (Pol I) promoter in transfected cells, an effect opposite to that observed on viral and cellular Pol II promoters. Nucleoli are thus, after PML bodies and centromeres, a novel nuclear structure targeted by ICP0.

摘要

本研究描述了单纯疱疹病毒1型的病毒蛋白ICP0的核仁定位。我们发现，ICP0的环指结构域对于ICP0定位在转染细胞和感染4小时的细胞的核仁中至关重要。ICP0形成了特定的核仁内结构域，这些结构域与任何已知的核仁结构域均不对应。通过对分级分离的感染细胞进行免疫印迹证实了这种分布。定量逆转录聚合酶链反应实验表明，ICP0不会增加转染细胞中RNA聚合酶I（Pol I）启动子的转录，这一效应与在病毒和细胞Pol II启动子上观察到的相反。因此，核仁是继早幼粒细胞白血病蛋白（PML）小体和着丝粒之后，被ICP0靶向的一种新的核结构。