高速逆流色谱法从秦皮中分离纯化香豆素类化合物
Isolation and purification of coumarin compounds from Cortex fraxinus by high-speed counter-current chromatography.
作者信息
Liu Renmin, Sun Qinghua, Sun Ailing, Cui Jichun
机构信息
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
出版信息
J Chromatogr A. 2005 Apr 29;1072(2):195-9. doi: 10.1016/j.chroma.2005.03.023.
High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of coumarin compounds from Cortex fraxinus, the Chinese herbal drug. n-Butanol-methanol-0.5% acetic acid (5:1.5:5, v/v) was used as the two-phase solvent system. 14.3 mg of fraxin, 26.5 mg of aesculin, 5.8 mg of fraxetin and 32.4 mg of aesculetin with the purity of 97.6, 99.5, 97.2 and 98.7%, respectively were obtained from 150 mg of crude extracts of C. fraxinus in a single run. The structures of the isolated compounds were identified by 1H NMR and 13C NMR.
高速逆流色谱法（HSCCC）成功用于从中药材秦皮中分离纯化香豆素类化合物。以正丁醇-甲醇-0.5%乙酸（5:1.5:5，v/v）作为两相溶剂体系。单次运行从150 mg秦皮粗提物中分别获得了纯度为97.6%的秦皮苷14.3 mg、纯度为99.5%的七叶苷26.5 mg、纯度为97.2%的秦皮素5.8 mg以及纯度为98.7%的七叶内酯32.4 mg。通过1H NMR和13C NMR鉴定了分离得到的化合物的结构。
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