School of Agriculture, Yangtze University, Jingzhou 434020, China.
Institute of Pesticides, Yangtze University, Jingzhou 434020, China.
Biomolecules. 2020 Jan 2;10(1):74. doi: 10.3390/biom10010074.
The phytochemical investigation of led to the isolation and characterization of ten compounds which were identified as fraxin (), fraxetin (), esculetin (), cichoriin (), euphorbetin (), kaempferol-3---rutinoside (), oleuropein (), linoleic acid (), methyl linoleate (), and -sitosterol (). Structures of the isolated constituents were characterized by H NMR, C NMR and HRMS. All the compounds, except compounds and were isolated for the first time from this plant. Further, this was the first report for the occurrence of compound in the species. Antifungal activity evaluation showed that compound exhibited significant inhibitory effects against and with EC values of 0.31 ± 0.01 mmol/L, 10.50 ± 0.02 mmol/L, and 0.40 ± 0.02 mmol/L respectively, compared to the positive control, Carbendazim, with its EC values of 0.74 ± 0.01 mmol/L, 1.78 ± 0.01 mmol/L and 1.41 ± 0.00 mmol/L. Herbicidal activity tests showed that compounds - had strong inhibitory effects against the roots of with EC values of 1.16 ± 0.23 mmol/L, 1.28 ± 0.58 mmol/L and 1.33 ± 0.35 mmol/L respectively, more potently active than that of the positive control, Cyanazine, with its EC values of 1.56 ± 0.44 mmol/L. However, none of the compounds proved to be active against the tested bacteria ( and ).
从 中分离得到了 10 种化合物，分别鉴定为秦皮素（）、秦皮素（）、七叶苷（）、菊苣苷（）、羟基-β-羽扇豆醇（）、山柰酚-3---芸香糖苷（）、橄榄苦苷（）、亚油酸（）、亚麻酸甲酯（）和 β-谷甾醇（）。通过 1H NMR、13C NMR 和 HRMS 对分离得到的化合物的结构进行了表征。除化合物 和 外，所有化合物均首次从该植物中分离得到。此外，这是首次在 种中发现化合物 。抗真菌活性评价表明，化合物 对 和 表现出显著的抑制作用，EC 值分别为 0.31 ± 0.01 mmol/L、10.50 ± 0.02 mmol/L 和 0.40 ± 0.02 mmol/L，与阳性对照剂多菌灵相比，其 EC 值分别为 0.74 ± 0.01 mmol/L、1.78 ± 0.01 mmol/L 和 1.41 ± 0.00 mmol/L。除草活性试验表明，化合物 - 对 根具有较强的抑制作用，EC 值分别为 1.16 ± 0.23 mmol/L、1.28 ± 0.58 mmol/L 和 1.33 ± 0.35 mmol/L，比阳性对照剂氰草津的 EC 值 1.56 ± 0.44 mmol/L 更有效。然而，没有一种化合物对测试的细菌（和 ）表现出活性。
