Bookjans G, Böger P
Z Naturforsch C Biosci. 1979 Aug;34(7-8):637-40. doi: 10.1515/znc-1979-7-826.
Ferredoxin-NADP+ reductase from the microalga Bumilleriopsis was isolated by a combination of affinity chromatography on a flavodoxin-Sepharose 4 B column and usual purification procedures. Both the elution pattern of the final gel filtration step and of the sodium dodecylsulfate (SDS) disc gel electrophoresis indicate that there are at least two different molecular-weight forms of the reductase, a monomeric form and a dimeric one.
通过在黄素氧还蛋白-琼脂糖凝胶4B柱上进行亲和层析与常规纯化程序相结合的方法,从微藻布氏藻中分离出了铁氧化还原蛋白-NADP⁺还原酶。最终凝胶过滤步骤和十二烷基硫酸钠(SDS)圆盘凝胶电泳的洗脱模式均表明,该还原酶至少有两种不同分子量的形式,一种是单体形式,另一种是二聚体形式。