昆虫激素和肽对中肠干细胞增殖与分化的刺激作用。

Stimulation of midgut stem cell proliferation and differentiation by insect hormones and peptides.

作者信息

Smagghe Guy, Vanhassel Wendy, Moeremans Clarina, De Wilde Daan, Goto Shintaro, Loeb Marcia J, Blackburn Michael B, Hakim Raziel S

机构信息

Laboratory of Agrozoology, Ghent University, Belgium.

出版信息

Ann N Y Acad Sci. 2005 Apr;1040:472-5. doi: 10.1196/annals.1327.094.

DOI:10.1196/annals.1327.094

PMID:15891093

Abstract

Stem cells derived from midguts of the caterpillar, Spodoptera littoralis, can be induced to multiply and differentiate in vitro. Ecdysone (E) and 20-hydroxyecdysone (20E) had a concentration-dependent effect: E was more active in cell proliferation and 20E in differentiation. Ecdysteroid receptors in midgut stem cell nuclei were stained with the antibody 9B9. In addition, alpha-arylphorin and four midgut differentiation factors (MDF) specifically stimulated proliferation and differentiation of stem cells, respectively. The activity of a panel of peptide growth factors and hormones on growth and metamorphosis of the insect midgut is discussed.

摘要

源自棉铃虫中肠的干细胞可在体外被诱导增殖和分化。蜕皮激素（E）和20-羟基蜕皮激素（20E）具有浓度依赖性效应：E在细胞增殖方面更具活性，而20E在分化方面更具活性。中肠干细胞核中的蜕皮甾体受体用抗体9B9进行染色。此外，α-芳基脂蛋白和四种中肠分化因子（MDF）分别特异性地刺激干细胞的增殖和分化。讨论了一组肽生长因子和激素对昆虫中肠生长和变态的活性。