Shimada S, Inagaki S, Narita N, Takagi H
Second Department of Anatomy, Osaka University Medical School, Suita, Japan.
Neurosci Lett. 1992 Jan 6;134(2):243-6. doi: 10.1016/0304-3940(92)90526-d.
An immunoelectron microscopic method combined with immunofluorescence double staining was carried out to examine the relationship between calcitonin gene-related peptide (CGRP)-like immunoreactive (LI) axon terminals and enkephalin (ENK)-LI neurons in the central amygdaloid nucleus (Ce) of the rat. The latter method showed that many ENK-LI cell bodies are densely surrounded by CGRP-LI axons in the lateral subdivision of the Ce (CeL). After taking fluorescence micrographs, the immunoperoxidase technique was used to examine the CGRP-LI axonal profiles under an electron microscope. CGRP-LI terminals were frequently found to form axo-somatic synaptic contacts with ENK-LI neurons in the CeL.
采用免疫电子显微镜方法并结合免疫荧光双重染色，来研究大鼠中央杏仁核（Ce）中降钙素基因相关肽（CGRP）免疫反应性（LI）轴突终末与脑啡肽（ENK）-LI神经元之间的关系。后一种方法显示，在Ce的外侧亚区（CeL）中，许多ENK-LI细胞体被CGRP-LI轴突密集包围。拍摄荧光显微照片后，使用免疫过氧化物酶技术在电子显微镜下检查CGRP-LI轴突轮廓。经常发现CGRP-LI终末在CeL中与ENK-LI神经元形成轴-体突触联系。
