Scheuner G, Flemming G
Acta Histochem. 1979;64(2):249-61.
In the paper a technique of the polarization optical qualitative and quantitative detection of polyanions with N,N'-diethylpseudoisocyaninchloride in the histological section is described. It is possible to prevent depolymerisation after precipitation with potassium ferricyanide and to intensify the birefringence of the histological structures. In vitro attempts and measurements of the dispersion of the double refraction and of the dichroism indicate connections between the optical appearances, furthermore it is possible to make statements about the mechanism of the arrangement of the tissue-dye-ferricyanide-complex.
本文描述了一种在组织切片中用氯化N，N'-二乙基假异氰尿酸对聚阴离子进行偏振光定性和定量检测的技术。在用铁氰化钾沉淀后可以防止解聚，并增强组织结构的双折射。体外对双折射和二色性色散的尝试和测量表明了光学现象之间的联系，此外，还可以对组织 - 染料 - 铁氰化物复合物的排列机制进行阐述。
