重组金丝雀痘病毒载体西尼罗河病毒（WNV）疫苗可保护犬猫免受蚊虫传播的西尼罗河病毒感染。

Recombinant canarypox vectored West Nile virus (WNV) vaccine protects dogs and cats against a mosquito WNV challenge.

作者信息

Karaca K, Bowen R, Austgen L E, Teehee M, Siger L, Grosenbaugh D, Loosemore L, Audonnet J-C, Nordgren R, Minke J M

机构信息

Merial Limited, 115 Transtech Drive, Athens, GA 30601, USA.

出版信息

Vaccine. 2005 May 31;23(29):3808-13. doi: 10.1016/j.vaccine.2005.02.020. Epub 2005 Mar 11.

DOI:10.1016/j.vaccine.2005.02.020

PMID:15893618

Abstract

The safety and efficacy of a canarypox vector expressing PrM and E genes of West Nile virus (WNV) (ALVAC-WNV) was evaluated in dogs and cats. One group of 17 dogs (vaccinated with 10(5.6) TCID(50)) and two groups of cats (groups 1 [n=14] vaccinated with 10(7.5) TCID(50) and 2 [n=8] 10(5.6) TCID(50)) were vaccinated twice at 28-day intervals. Fifteen dogs and eleven cats served as negative controls. The cats and dogs were challenged 120 and 135 days after the second immunization, respectively via the bites of Aedes albopictus mosquitoes infected with WNV. The first dose of vaccine induced a detectable antibody response in four dogs and five cats (one immunized with low and four with high doses). After the second dose, all the vaccinated dogs and all of the cats, immunized with high dose had detectable antibody titers, whereas only four of eight cats in the low dose group were seropositive. None of the vaccinated dogs and one vaccinated cat developed viremia following the WNV mosquito-challenge. In contrast, 14 of the 15 control dogs and 9 of the 11 control cats developed viremia. The experimental vaccine described in this study may be of value in the prevention of WNV infection in dogs and cats.

摘要

在犬类和猫类动物中评估了表达西尼罗河病毒（WNV）前体膜蛋白（PrM）和包膜蛋白（E）基因的金丝雀痘病毒载体（ALVAC-WNV）的安全性和有效性。一组17只犬（接种10(5.6)半数组织培养感染剂量（TCID(50)））和两组猫（第1组[n = 14]接种10(7.5) TCID(50)，第2组[n = 8]接种10(5.6) TCID(50)）每隔28天接种两次。15只犬和11只猫作为阴性对照。在第二次免疫后120天和135天，分别通过感染WNV的白纹伊蚊叮咬对犬和猫进行攻毒。第一剂疫苗在4只犬和5只猫（1只低剂量免疫和4只高剂量免疫）中诱导出可检测到的抗体反应。第二次接种后，所有接种疫苗的犬以及所有高剂量免疫的猫都有可检测到的抗体滴度，而低剂量组8只猫中只有4只血清呈阳性。接种疫苗的犬和1只接种疫苗的猫在WNV蚊虫攻毒后均未出现病毒血症。相比之下，15只对照犬中有14只和11只对照猫中有9只出现了病毒血症。本研究中描述的实验性疫苗可能对预防犬类和猫类动物感染WNV具有价值。