男性血浆中游离及结合雌激素的连续放射免疫测定

Sequential radioimmunoassay of unconjugated and conjugated estrogen in male human plasma.

作者信息

Ohashi T, Saito T, Ohmori H

出版信息

Acta Med Okayama. 1979 Jun;33(3):157-66.

Abstract

A sequential radioimmunoassay procedure for unconjugated and conjugated estrone, estradiol-17 beta and estriol in male human plasma was developed. The blank values in this assay for unconjugated estrone, estradiol-17 beta, estriol conjugated estrone, estradiol-17 beta and conjugated estriol were 0.36 +/- 1.14 pg, 3.90 +/- 2.75 pg, 2.25 +/- 2.08 pg, 0.92 +/- 1.51 pg, 5.02 +/- 2.86 pg and 3.12 +/- 2.97 pg, respectively. Mean values of unconjugated estrone, estradiol-17 beta, estriol, conjugated estrone, estradiol-17 beta and conjugated estriol in plasma from 28 normal adult males were 38.4 +/- 13.4 pg/ml, 32.6 +/- 9.90 pg/ml, 4.06 +/- 3.68 pg/ml, 34.2 +/- 13.8 pg/ml, 40.4 +/- 12.3 pg/ml and 31.8 +/- 7.41 pg/ml, respectively. Both unconjugated and conjugated estrogen levels in patients with liver cirrhosis were elevated and conjugated estrogen, especially estriol levels, in patients with renal insufficiency were markedly elevated.

摘要

建立了一种用于检测男性人血浆中游离型和结合型雌酮、雌二醇 - 17β 和雌三醇的连续放射免疫分析方法。该分析中游离型雌酮、雌二醇 - 17β、雌三醇、结合型雌酮、雌二醇 - 17β 和结合型雌三醇的空白值分别为 0.36±1.14 pg、3.90±2.75 pg、2.25±2.08 pg、0.92±1.51 pg、5.02±2.86 pg 和 3.12±2.97 pg。28 名正常成年男性血浆中游离型雌酮、雌二醇 - 17β、雌三醇、结合型雌酮、雌二醇 - 17β 和结合型雌三醇的平均值分别为 38.4±13.4 pg/ml、32.6±9.90 pg/ml、4.06±3.68 pg/ml、34.2±13.8 pg/ml、40.4±12.3 pg/ml 和 31.8±7.41 pg/ml。肝硬化患者的游离型和结合型雌激素水平均升高，肾功能不全患者的结合型雌激素，尤其是雌三醇水平显著升高。