鸡生长因子的部分纯化及其生物学活性与特性

Partial purification and biological activities and properties of chick growth factors.

作者信息

Takahashi F, Kuramitsu M, Tokuda M, Matsui H, Itano T, Murakami T H, Hatase O, Nisida I

出版信息

Acta Med Okayama. 1979 Jun;33(3):167-76.

Abstract

Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.

摘要

对鸡胚 carcasses 和成年肌肉上清液中促进细胞增殖的细胞刺激因子进行了研究。胚胎和成年肌肉上清液中存在多种刺激因子，无需添加血清和其他物质就能促进细胞增殖。采用硫酸铵盐析法、乙醇分级分离法和等电沉淀法分离刺激因子，这三种方法均证明鸡胚和成年肌肉上清液中存在多种促进细胞增殖的刺激物。由于胚胎发育，这些刺激物的物理化学性质和生物学活性发生了改变。胚胎刺激物显著增强了 DNA 和蛋白质的合成，在全细胞系统中对 RNA 合成的促进作用较小。肌肉刺激物增强了蛋白质合成，但对 DNA 和 RNA 合成没有任何刺激作用。通过 DEAE - 纤维素色谱法和 Sephadex 凝胶色谱法对乙醇级分中的刺激物进行了部分纯化。